Sanger sequencing data analysis software tools
Since its development in the late 1970s, Sanger chain-termination DNA sequencing (Sanger et al., 1977) has become a widely used, essential technique of molecular biology. Although high-coverage, high-volume sequencing has largely moved to “next-generation” technologies, Sanger sequencing remains a popular and indispensable tool for low-coverage sequencing applications
(Kircher and Kelso 2010) High-throughput DNA sequencing--concepts and limitations. Bioessays.