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Sanger sequencing software tools

Since its development in the late 1970s, Sanger chain-termination DNA sequencing (Sanger et al., 1977) has become a widely used, essential technique of molecular biology. Although high-coverage, high-volume sequencing has largely moved to “next-generation” technologies, Sanger sequencing remains a popular and indispensable tool for low-coverage sequencing applications (Kircher and Kelso, 2010).