SAPAS protocols

SAPAS specifications

Information


Unique identifier OMICS_01413
Name SAPAS
Software type Pipeline/Workflow
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Perl
Computer skills Advanced
Stability Beta
Maintained Yes

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Publication for SAPAS

SAPAS IN pipelines

 (2)
2015
PMCID: 4384636
PMID: 25838129
DOI: 10.7554/eLife.05003.028

[…] approaches; standard whole mrna sequencing (rnaseq) and a sequencing method, 3pseq, that captures specifically the sequence adjacent to the poly(a) tail. our 3pseq protocol is similar to the sapas method described previously (fu et al., 2011). in contrast to sapas, total mrna was fragmented chemically, resulting in 200 nucleotide long molecules. cdna was generated and amplified using […]

2015
PMCID: 4384636
PMID: 25838129
DOI: 10.7554/eLife.05003.028

[…] method, 3pseq, that captures specifically the sequence adjacent to the poly(a) tail. our 3pseq protocol is similar to the sapas method described previously (fu et al., 2011). in contrast to sapas, total mrna was fragmented chemically, resulting in 200 nucleotide long molecules. cdna was generated and amplified using a polyt primer terminating with a dinucleotide made of non-t followed […]

SAPAS institution(s)
Institute of Medical Biostatistics, Epidemiology and Informatics, University Medical Center Johannes Gutenberg University Mainz, Mainz, Germany; Statistical Bioinformatics, Bioinformatics Core Facility, University Medical Center Johannes Gutenberg, Mainz, Germany; Molecular Cardiology Genomics and System Biology group, Department of General and Interventional Cardiology, University Heart Center Hamburg-Eppendorf, Hamburg, Germany; Division Biostatistics and Bioinformatics, University Medical Center Johannes Gutenberg University Mainz, Mainz, Germany; Institute of Medical Biostatistics, Epidemiology and Informatics, University Medical Center Johannes Gutenberg, Mainz, Germany

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