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Protocols

SASqPCR specifications

Information


Unique identifier OMICS_02375
Name SASqPCR
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Publication for SASqPCR

SASqPCR citations

 (5)
library_books

LEMming: A Linear Error Model to Normalize Parallel Quantitative Real Time PCR (qPCR) Data as an Alternative to Reference Gene Based Methods

2015
PLoS One
PMCID: 4556681
PMID: 26325269
DOI: 10.1371/journal.pone.0135852

[…] A basic technique for normalization is the ΔΔC t method described by Livak and Schmittgen [] and Pfaffl []. The ΔΔC t method relies on a single RG. Current tools like qBase+ [], DAG Expression [] and SASqPCR [] use multiple RGs for normalization. They implement a normalization strategy that originates from Vandesompele et al [], who suggested the use of multiple RGs. Their tool geNorm allows the m […]

call_split

The effect of immunological status, in vitro treatment and culture time on expression of eleven candidate reference genes in bovine blood mononuclear cells

2015
BMC Immunol
PMCID: 4449592
PMID: 26025301
DOI: 10.1186/s12865-015-0099-7
call_split See protocol

[…] Statistical analysis of the amplification performance was carried out using SASqPCR macro code in SAS STAT software (Ver. 9.3) []. The relative expression of each gene was calculated based on equation published by Pfaffl in 2001 []. Briefly, the first time point was considere […]

library_books

A survey of tools for the analysis of quantitative PCR (qPCR) data

2014
PMCID: 5129434
PMID: 27920994
DOI: 10.1016/j.bdq.2014.08.002

[…] ased on qBase , and the application is able to perform relative quantification and determination of efficiencies using standard curve calculation. Quantification results can be displayed as bar plots.SASqPCR is a program that requires the commercial SAS software suite, but according to the authors extensive SAS programming knowledge is not required. The tool performs calculations on Cq values and […]

library_books

Identification of Chiari Type I Malformation subtypes using whole genome expression profiles and cranial base morphometrics

2014
BMC Med Genomics
PMCID: 4082616
PMID: 24962150
DOI: 10.1186/1755-8794-7-39

[…] me). In order to identify appropriate endogenous controls to use for RT-qPCR, Human Taqman Endogenous Control Arrays (Applied Biosystems, Grand Island, NY) were run using blood and dura cDNA samples. SASqPCR [] was used to identify the most stable endogenous control genes. Due to sample limitations, only the top two most stable genes (lowest M value) were selected from the blood and dura RT-qPCR r […]

library_books

Accumulation of amyloid like Aβ1–42 in AEL (autophagy–endosomal–lysosomal) vesicles: potential implications for plaque biogenesis

2014
PMCID: 4379859
PMID: 24521233
DOI: 10.1042/AN20130044

[…] l, Cyp1, Elav, eIF-1A, l (3)02640, Rap2l, RpL32, Su (Tpl) and αTub84B] were measured for data-specific normalization (Ling and Salvaterra, ). CT values from real-time PCR were analyzed using a custom SASqPCR program (Ling, ). Mean normalized expression ratios were calculated as described (Ling et al., ). […]


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SASqPCR institution(s)
Department of Neuroscience, Beckman Research Institute of City of Hope, Duarte, CA, USA

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