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ScreenMill specifications


Unique identifier OMICS_27891
Name ScreenMill
Restrictions to use None
Community driven No
Data access Browse
User data submission Not allowed
Registration required Yes
Maintained No

Publication for ScreenMill

ScreenMill citations


Phenotypic landscape of non conventional yeast species for different stress tolerance traits desirable in bioethanol fermentation

Biotechnol Biofuels
PMCID: 5597992
PMID: 28924451
DOI: 10.1186/s13068-017-0899-5

[…] ion was measured by quantifying the spot size. This was achieved by scanning images of the test plates and analyzing the images using a dedicated image processing software ImageJ [] combined with the ScreenMill plugin []. Next, relative growth (RG) of each strain in a specific test condition was calculated by dividing the actual growth of the strain on that test plate by the growth of that strain […]


Early onset torsion dystonia: a novel high throughput yeast genetic screen for factors modifying protein levels of torsinAΔE

PMCID: 5611967
PMID: 28768697
DOI: 10.1242/dmm.029926
call_split See protocol

[…] re visualized using a Kodak 440CF Image Station, a Bio-Rad ChemiDoc XRS+ or an Amersham Imager 600 (GE Healthcare). The signal was quantified using ImageJ v1.46r (NIH, USA). A modified version of the ScreenMill software suite () that allows for quantification of normalized colony-blot signal (Fig. S1B, Table S1) was used to quantify the signal in ImageJ (Appendix S1). The background subtracted mod […]


Domestication and Divergence of Saccharomyces cerevisiae Beer Yeasts

PMCID: 5018251
PMID: 27610566
DOI: 10.1016/j.cell.2016.08.020

[…] After 4-14 days of incubation (depending on the experiment), all plates were scanned using a high-definition scanner (Seiko Epson, Japan). Scanned images were processed using ImageJ combined with the ScreenMill macro (). Data were processed by calculating relative growth compared to the control condition, and subsequent normalization by conversion to z-scores (). Heat maps were obtained using the […]


Genome wide Protein protein Interaction Screening by Protein fragment Complementation Assay (PCA) in Living Cells

PMCID: 4401175
PMID: 25867901
DOI: 10.3791/52255

[…] Analyze images of colony arrays with custom ImageJ31 scripts or using published softwares such as Colonizer, Ht colony grid analyzer, Cell profiler, Colony Imager, ScreenMill, YeastXtract and gitter (compiled in 32). Image analysis should output one or several spreadsheets containing colony sizes for each position of each array, use these colony sizes for all do […]


Large scale robot assisted genome shuffling yields industrial Saccharomyces cerevisiae yeasts with increased ethanol tolerance

Biotechnol Biofuels
PMCID: 4354739
PMID: 25759747
DOI: 10.1186/s13068-015-0216-0

[…] using an Epson Perfection V700 PHOTO at 300 dpi at regular intervals, typically for up to 2 to 3 weeks. YPD plates (control) were scanned after two days. The scans were quantified in ImageJ using the ScreenMill software suite operated in summation mode []. For screening natural strains, ethanol tolerance was expressed as the end point area on medium with ethanol normalized by the area on YPD. For […]

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ScreenMill institution(s)
Columbia University, Department of Biological Sciences, New York, NY, USA; Columbia University Medical Center, Department Genetics & Development, New York, NY, USA
ScreenMill funding source(s)
Supported by the National Institutes of Health grants CA125520, GM50237, and HG01620 awarded, CA009503, GM008798 and HG00193.

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