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SeqBuilder Pro specifications


Unique identifier OMICS_32566
Name SeqBuilder Pro
Alternative name SeqBuilder
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Mac OS, Windows
Computer skills Medium
Stability Stable
Free trial Yes
Maintained Yes


No version available



  • person_outline SeqBuilder Pro Team

SeqBuilder Pro citations


TIA: algorithms for development of identity linked SNP islands for analysis by massively parallel DNA sequencing

BMC Bioinformatics
PMCID: 5896139
PMID: 29642839
DOI: 10.1186/s12859-018-2133-2

[…] Primer sets were computationally designed for the unique sequences of the low-variance SNP island flanking regions using the SeqBuilder module of the DNASTAR software suite (version During the searches, primer length range was set to between 25 and 45 bp with a target annealing temperature of 70 °C. Identified p […]


The complete mitochondrial genome of Somanniathelphusa boyangensis and phylogenetic analysis of Genus Somanniathelphusa (Crustacea: Decapoda: Parathelphusidae)

PLoS One
PMCID: 5810993
PMID: 29438407
DOI: 10.1371/journal.pone.0192601

[…] re submitted to the NCBI database. Based on the positions of the tRNAs and rRNAs, the approximate locations of 13 protein-coding genes (PCGs) were calculated, and PCG annotations were performed using SeqBuilder software (DNAStar)[]. The secondary structure of the tRNA genes was putatively predicted using the web server ARWEN[]. Gene maps of the mitochondrial genome were constructed using the web s […]


Identification and characterization of influenza A viruses in selected domestic animals in Kenya, 2010 2012

PLoS One
PMCID: 5806879
PMID: 29425232
DOI: 10.1371/journal.pone.0192721

[…] ed in the phylogenetic analysis is provided ().HA nucleotide contigs were assembled using the SeqMan Pro of Lasergene 11 software (DNASTAR, Inc, Madison, WI, US) and protein translations were done in Seqbuilder of Lasergene 11 software (DNASTAR, Inc, Madison, WI, US). Multiple sequence alignments were performed in BioEdit []. Phylogenetic analyses were performed with MrBayes 3.1 []. The generalize […]


Whole genome resequencing identifies the CPQ gene as a determinant of ascites syndrome in broilers

PLoS One
PMCID: 5749702
PMID: 29293530
DOI: 10.1371/journal.pone.0189544

[…] Specific genomic sequences from the Gallus_gallus-5 assembly were manipulated and annotated in SeqBuilder software (DNASTAR Lasergene Suite 14). PCR primers and probes were designed using Primer3 (version 0.4.0; using custom primer size (19-30bp), GC […]


Transcription coupled repair and biased insertion of human retrotransposon L1 in transcribed genes

PMCID: 5717806
PMID: 29225704
DOI: 10.1186/s13100-017-0100-5
call_split See protocol

[…] nologies). Cloned PCR products were Sanger sequenced using M13 forward and reverse primers. Samples were sent for Sanger sequencing to Elim Biopharmaceuticals, Inc., Hayward, California. Lasergene 10 SeqBuilder software was utilized for sequence analysis and the sequences were compared to the reference cDNA using BLAST software (website: […]


Complete Genome Sequence of Lactobacillus plantarum 10CH, a Potential Probiotic Lactic Acid Bacterium with Potent Antimicrobial Activity

Genome Announc
PMCID: 5722076
PMID: 29192090
DOI: 10.1128/genomeA.01398-17
call_split See protocol

[…] oneAmp HiFi PCR premix (TaKaRa, Japan). The resulting amplicons were analyzed by gel electrophoresis and sequenced by Eurofins Genomics. The sizes of the amplified gaps ranged from 900 bp to ~5.5 kb. SeqBuilder (Lasergene) was used to fill the gaps using the resulting sequences of the amplified PCR products.The sequencing results were consistent with the presence of a single circular replicon of 3 […]


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SeqBuilder Pro institution(s)
DNASTAR, Inc., Madison, WI, USA

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