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Permits users to analyse small RNA data from Illumina sequencing. SeqBuster is a web-based bioinformatic tool providing an automatized pre-analysis for sequence annotation. This software offers a custom analysis of deep sequencing data at different levels, with special emphasis on the analysis of miRNA variants or isomiRs. It contains an analysis module to explore the miRNA targets using the miRBase, PicTar and/or TargetScan algorithms.

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SeqBuster classification

SeqBuster specifications

Software type:
Restrictions to use:
GNU General Public License version 2.0
Command line interface
Operating system:
Computer skills:


  • miraligner
  • SeqCluster

SeqBuster distribution


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SeqBuster support



  • Eulalia Martí <>
  • Xavier Estivill <>

Additional information


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Genetic Causes of Disease Group, Genes and Disease Program, Centre for Genomic Regulation (CRG), Barcelona, Spain; Centro de Investigacion Biomedica en Red en Epidemiologıa y Salud Publica (CIBERESP), Barcelona, Spain; Experimental and Health Sciences Department, Pompeu Fabra University, Barcelona, Catalonia, Spain

Funding source(s)

Supported by Spanish Ministry of Health Fondo de Investigaciones Sanitarias (PI081367) and Instituto de Salud Carlos III (CIBERESP), Spanish Ministry of Science and Innovation (SAF2008-00357) the Sixth Framework Programme of the European Commission through the SIROCCO integrated project LSHG-CT-2006-037900, the Spanish Ministry of Science and Innovation (SAF2008-00357), the Spanish Ministry of Health and a fellowship from the Spanish Ministry of Science and Innovation MICINN.

Link to literature

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