SerialEM statistics

info info

Citations per year

info

Popular tool citations

chevron_left 3D macromolecule reconstruction Image acquisition Serial block face imaging 3D reconstruction Image acquisition chevron_right
info

Tool usage distribution map

Tool usage distribution map
info info

Associated diseases

Associated diseases
Want to access the full stats & trends on this tool?

Protocols

SerialEM specifications

Information


Unique identifier OMICS_15825
Name SerialEM
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input format XML
Output format XML
Operating system Windows
Computer skills Medium
Version 3.6
Stability Stable
Source code URL http://bio3d.colorado.edu/ftp/SerialEM/
Maintained Yes

Download


download.png

Versioning


No version available

Maintainer


  • person_outline David Mastronarde <>

Publications for SerialEM

SerialEM citations

 (262)
library_books

Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin independent endocytosis

2018
PMCID: 5943408
PMID: 29743604
DOI: 10.1038/s41467-018-03955-w

[…] 4k lens coupled ccd camera (direct electron) and a tilt rotate holder (fischione) utilising a tilt range of −60 to +60°. microscope control and image acquisition was accomplished using the software serialem. tilt series were later reconstructed using weighted back projection and fiducial markers (10 nm) into a single volume with imod. to examine areas with the greatest electron density […]

library_books

Promotion of virus assembly and organization by the measles virus matrix protein

2018
PMCID: 5928126
PMID: 29712906
DOI: 10.1038/s41467-018-04058-2

[…] nominal magnification) or 6.14 å (10,000× nominal magnification) on the level of the specimen. bidirectional tilt series were semi-automatically collected with 2° angular increments using the serialem package. a cumulative electron dose between 120 and 140 e− å−2 was used. all frames were motion corrected and damage compensated using python scripts (de_process_frames-2.8.1.py […]

library_books

Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification

2018
PMCID: 5919621
PMID: 29698455
DOI: 10.1371/journal.pone.0196632

[…] at 300 kv, with a gatan k2 summit electron detection (ded) camera (gatan, pleasanton, ca) in super resolution mode. images were then binned down 2x to achieve a pixel size of 2.635 å, using serialem version 3.6 []. tilt series were collected using a bidirectional tilt scheme from 0°±60° with an increment of 1.5° and a defocus range of -5.0 to -6.0 μm. all identified mitochondria […]

library_books

Cryo EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins

2018
PMCID: 5933801
PMID: 29684066
DOI: 10.1371/journal.ppat.1007009

[…] were recorded using a gatan k2 summit direct electron detector in the direct electron counting mode (gatan), attached to a fei titan-krios tem, at arizona state university. the automated software serialem [] was used to collect ~2,000 total movies at 37,700x magnification and at a defocus range between 1 and 3 μm. each movie had a total accumulated exposure of 53.66 e/å2 fractionated in 50 […]

library_books

Ultrastructural localisation of protein interactions using conditionally stable nanobodies

2018
PMCID: 5903671
PMID: 29621251
DOI: 10.1371/journal.pbio.2005473

[…] ultrathin sections were cut, placed on a slot grid, and imaged on a tecnai 12 transmission electron microscope fitted with a 4k x 4k lc1100 camera (direct electron) at 120 kv under the control of serialem. low-magnification (4,400 xmag) montages were acquired at a binning of 1 and stitched together using the blendmont program in imod. correlation of light and em images was performed using […]

library_books

Clathrin adaptor ratio and membrane tension regulate the flat to curved transition of the clathrin coat during endocytosis

2018
PMCID: 5856840
PMID: 29549258
DOI: 10.1038/s41467-018-03533-0

[…] then carefully placed on a glow discharged em grid. samples were dried on filter paper and again imaged with a phase contrast microscope. tem imaging was performed using a jeol 1400 equipped with serialem freeware for montaging. montages of large membrane sheets at 12,000 magnification (1.82 nm per pixel) with 10% overlap were imaged., the fm image and the em montage of the same membrane […]


Want to access the full list of citations?
SerialEM institution(s)
Boulder Laboratory for Three-Dimensional Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA
SerialEM funding source(s)
This work was supported by NIH/NCRR Grant RR00592 and NIH Program Project Grant P01GM61306.

SerialEM reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review SerialEM