sgRNAcas9 statistics

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sgRNAcas9 specifications

Information


Unique identifier OMICS_04862
Name sgRNAcas9
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Windows
Programming languages Perl
License GNU General Public License version 2.0
Computer skills Advanced
Stability Stable
Maintained Yes

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Publication for sgRNAcas9

sgRNAcas9 in publications

 (11)
PMCID: 5943413
PMID: 29743485
DOI: 10.1038/s41598-018-25366-z

[…] genes in each of these genomes. here we illustrate how we predicted the grnas in protein-coding genes in dmku 3-1042. for each protein-coding gene, we used its sequence as the input of sgrnacas9 (version 2.0.7) to predict the grnas with sequences with ngg protospacer adjacent motif (pam) and without off-target. the off-target of a grna is defined as a genomic site with <six […]

PMCID: 5930475
PMID: 29725264
DOI: 10.7150/ijbs.23890

[…] institutional animal care and use committee of inner mongolia university. all animals were maintained at the inner mongolia yiwei white cashmere goat limited liability company., using the free tool sgrnacas9 to analyze the coding sequence of edar, two sgrnas were designed for targeting exon 6: sgrna1 (5′-ggagaacttctccgcggggc-3′) and sgrna2 (5′-cggcgccacaaggactgcga-3′). two sets of sgrna […]

PMCID: 5778067
PMID: 29358698
DOI: 10.1038/s41598-018-19774-4

[…] length of time from fertilization to the first cleavage in rabbit embryos than in mouse embryos. early injection may provide sufficient time for cas9rna translation and prolong the exposure to the sgrnacas9 complex and ssodns before the first cleavage. the results suggested that adjustment of the interval between the hcg processing time and rna injection time may affect the efficiency […]

PMCID: 5919725
PMID: 29233915
DOI: 10.1534/g3.117.1134

[…] to 1 μg/μl, aliquoted, and stored at 80° until use. three possible off-target sites of each sgrna in the different mosquito species were identified based on the chopchopv2 software () and local sgrnacas9 package (), and analyzed by using a t7 endonuclease i (t7ei) assay, respectively. briefly, genomic dna was extracted from mosquitoes with the dneasy blood & tissue kit (qiagen) […]

PMCID: 5209885
PMID: 28053662
DOI: 10.1186/s13068-016-0693-9

[…] number: m16371.1), cre-1 (mycth_2310085), res-1 (mycth_2302052), gh1-1 (mycth_115968), and alp-1 (mycth_2303011), all sgrna target sites in the genome of m. thermophila were identified using the sgrnacas9 tool []. sgrna target sites with high scores were chosen and the corresponding oligos were ordered (additional file : table s1). all protospacer sequences used to target the five different […]


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sgRNAcas9 institution(s)
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, China

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