sgTiler specifications


Unique identifier OMICS_23745
Name sgTiler
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input data A bowtie index file for the desired genome, a file containing genome exon regions and an open chromatin or any histone mark.
Input format FASTA+BED+BED
Output data A list of all candidate sgRNAs, a list of filtered sgRNAs, a list of sgRNA details for the input sequences, a summary report with important statistics, a file with graphical summaries of no. of sgRNAs and a sequence coverage per input region.
Output format TXT+PDF
Operating system Unix/Linux, Mac OS, Windows
Programming languages Python
Computer skills Advanced
Stability Stable
Maintained Yes




No version available


  • person_outline Hansen He

Publication for sgTiler

sgTiler institution(s)
Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada; Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
sgTiler funding source(s)
Supported by PMCF, CFI and ORF, NSERC (498706), WICC and CCS (703800), CCSRI (702922), PCC (RS2016-02; RS2014-04), CIHR (142246), NCI at the NIH (R01CA155004; LM009012 and LM010098) and a young investigator award from the OICR and a new investigator salary award from the CIHR.

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