Signal-3L

[…] database using blastx (e-value ≤ 1e-5). the coding dna sequence (cds) and deduced amino acid sequence were obtained using open reading frame finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html). signal-3l was used to identify signal peptides and predgpi predictor to identify glycosylphosphatidylinositol (gpi) domains. oligonucleotide primers were designed according to thornton and basu […]

[…] replicates. the analysis was conducted with mega 5.01 []., prediction of subcellular localization, signal peptide and transmembrane domains were conducted using default settings of suba 3 [], signal-3l [] and tmhmm [], respectively. expression profiles for mwl-1 and 2, across different developmental stages, was extracted from the bio-analytic resource for plant biology […]

[…] signal-blast [] analysis indicated that the first 19, 20 and 19 aa of the n-termini of cdta, cdtb and cdtc, respectively are signal peptides. both predsi (http://www.predisi.de/home.html) and signal-3l [] (http://www.csbio.sjtu.edu.cn/bioinf/signal-3l/) analysis indicate that the first n-terminal 19 aa and of cdta and cdtc, and 21 aa of cdtb are signal peptides ()., based on the signal […]

[…] database using blastx (e-value ≤ 1e-5). the coding dna sequence (cds) and deduced amino acid sequence were obtained using open reading frame finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html). signal-3l was used to identify signal peptides and predgpi predictor to identify glycosylphosphatidylinositol (gpi) domains. oligonucleotide primers were designed according to thornton and basu […]

[…] homology), mdh1 (97% homology), mdh2 (94% homology), ssu1 (89% homology), and ssu2 (68% homology). for the malate dehydrogenases n-terminal targeting sequences were analyzed using a combination of signal-3l and targetp 1.1 (). the putative localization for mdh1 was the cytosol and for mdh2 the mitochondrion. the n-terminal mitochondrial targeting sequence for mdh2 was either retained […]

[…] protein for transportation out of the cytoplasm in prokaryotes (). many methods have been used for predicting signal peptides, including signalp (; ; ; ), predisi (), speplip (), signal-cf (), signal-3l (), signal-blast (), phobius (), lipop () and philius (). all the prediction methods have limited ability to discriminate between signal peptides and n-terminal transmembrane helices. […]

[…] spoctopus (http://octopus.cbr.su.se/; cleavage at g58), speplip (http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi; cleavage at a59), signal3l (http://www.csbio.sjtu.edu.cn/bioinf/signal-3l/; cleavage at a30), emboss sigcleave (http://www.bioinformatics.nl/cgi-bin/emboss/sigcleave; cleavage at a32), and signalp (http://www.cbs.dtu.dk/services/signalp/; spi signal sequence […]