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chevron_left Single molecule detection and localization Particle tracking FLIM data analysis FCS data analysis Image simulation Image acquisition chevron_right
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SimFCS specifications

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Unique identifier OMICS_18387
Name SimFCS
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Windows
Computer skills Advanced
Version 4.0
Maintained No

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SimFCS in publications

 (72)
PMCID: 5801303
PMID: 29456966
DOI: 10.3389/fonc.2018.00013

[…] hp laser (spectra-physics). the microscope is coupled to a fluorescence correlation spectrometer alba flim (iss, inc.) and data were collected by vistavision software (iss, inc.) and analyzed by simfcs 4 software (). the samples were excited by two photons at 740 nm using a 60×/1.20 plan-apochromat water immersion objective lens (nikon). the emission was detected on an avalanche photodiode […]

PMCID: 5831029
PMID: 29507905
DOI: 10.1002/hep4.1139

[…] a zeiss 780 laser‐scanning confocal/multiphoton‐excitation fluorescence microscope, as described in the supporting information. phasor transformation and data analysis were carried out using global simfcs (laboratory for fluorescence dynamics, university of california, irvine) as described. fractional analysis of free versus bound nadh in each pixel was determined based on fluorescence lifetime […]

PMCID: 5773500
PMID: 29348472
DOI: 10.1038/s41598-018-19582-w

[…] the fluctuations of fluorescence intensity in a pixel over time in space to visualize the transition time of the particle. carpet analysis, acf and pcf calculations were carried out using simfcs (laboratory of fluorescence dynamics) and matlab (the mathworks inc., 2010). the line scan acf data for each pixel was fitted using a matlab routine to single component fcs equation to find […]

PMCID: 5768877
PMID: 29335600
DOI: 10.1038/s41598-018-19160-0

[…] lambda mode 32 channels from 416 nm to 728 nm and a bandwidth of 9.78 nm. the frame resolution was set to 256 × 256 pixels and with a pixel dwell time of 12.61μs/pixel. all data were analyzed using simfcs software available at (www.lfd.uci.edu). all cells were maintained at 37 °c and 5% co2 during imaging., here we employed an elegant way to separate fluorescently tagged protein signal […]

PMCID: 5760106
PMID: 29281723
DOI: 10.1371/journal.ppat.1006793

[…] fluorescence intensity time traces were acquired with 5-μs temporal resolution, and a 10-second-long auto-correlation function (acf; average ± sem) was subsequently computed by means of simfcs software (developed by enrico gratton, laboratory for fluorescence dynamics). globals for spectroscopy (developed by prof. gratton) was employed for the non-linear fit of the acfs to either […]

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