SimFCS statistics

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Citations per year

Number of citations per year for the bioinformatics software tool SimFCS

Tool usage distribution map

This map represents all the scientific publications referring to SimFCS per scientific context
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Associated diseases

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Popular tool citations

chevron_left Single molecule detection and localization Particle tracking FLIM data analysis FCS data analysis Image simulation Image acquisition chevron_right
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SimFCS specifications


Unique identifier OMICS_18387
Name SimFCS
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Windows
Computer skills Advanced
Version 4.0
Maintained No


No version available


This tool is not maintained anymore.

SimFCS citations


Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis

Sci Rep
PMCID: 5768877
PMID: 29335600
DOI: 10.1038/s41598-018-19160-0

[…] e lambda mode 32 channels from 416 nm to 728 nm and a bandwidth of 9.78 nm. The frame resolution was set to 256 × 256 pixels and with a pixel dwell time of 12.61μs/pixel. All data were analyzed using SimFCS software available at ( All cells were maintained at 37 °C and 5% CO2 during imaging. […]


Dextran Catechin: An anticancer chemically modified natural compound targeting copper that attenuates neuroblastoma growth

PMCID: 5216955
PMID: 27374085
DOI: 10.18632/oncotarget.10201

[…] er power at the sample was maintained at the mW level. The FLIM data were acquired with the MT200 software developed by Dr. Ales Benda and the phasor transformation and data analysis processed by the SimFCS software developed at Laboratory for Fluorescence Dynamics ( Calibration of the system and phasor plot space was performed by measuring Atto425, which has a known single expone […]


Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy

PMCID: 4946880
PMID: 27288545
DOI: 10.7554/eLife.14770.039

[…] over sampled (see RICS). In addition, lines expressing monomeric forms of GFP (35S:GFP) and mCherry (UBQ10:mCherry) were used to set the background fluorescence and to measure monomer brightness (see SimFCS Software Analysis).We obtained both the mean and the variance of the intensity distribution at each pixel in order to determine the number (N) and brightness (B) of the particles. The mean, […]


Spontaneous membrane translocating peptides: influence of peptide self aggregation and cargo polarity

Sci Rep
PMCID: 4645181
PMID: 26567719
DOI: 10.1038/srep16914
call_split See protocol

[…] olution and in cells. Typically, a ROI of 256 × 256 pixels (corresponding to an area of 6.4 × 6.4 μm) was selected and 200 total frames were collected. RICS correlation function was calculated by the SimFCS software, developed at the Laboratory for Fluorescence Dynamics ( Fitting of RICS function to extract the diffusion coefficient (D, μm2/s) of the dye-labeled peptide was perfor […]


Spatiotemporal regulation of Heterochromatin Protein 1 alpha oligomerization and dynamics in live cells

Sci Rep
PMCID: 4523856
PMID: 26238434
DOI: 10.1038/srep12001

[…] being sampled every 100 nm (32 pixels to a line). The average laser power at the sample for both frame and line scan acquisitions was maintained at the 1–2 mW. The data acquired were processed by the SimFCS software developed at the Laboratory for Fluorescence Dynamics ( […]


Investigating the Cellular Distribution and Interactions of HIV 1 Nucleocapsid Protein by Quantitative Fluorescence Microscopy

PLoS One
PMCID: 4344342
PMID: 25723396
DOI: 10.1371/journal.pone.0116921
call_split See protocol

[…] objective (NA = 1.2). eGFP fluorescence was excited with a 488 nm argon laser line and the emitted fluorescence (500–550 nm) was detected by a PMT detector. The RICS analysis was performed using the SimFCS software developed by the Laboratory for Fluorescence Dynamics ( For each cell, a stack of 50 images (256×256 pixels with a pixel size of 50 nm) was acquired at 400 Hz ( […]

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