simFRAP statistics

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Citations per year

Number of citations per year for the bioinformatics software tool simFRAP

Tool usage distribution map

This map represents all the scientific publications referring to simFRAP per scientific context
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Associated diseases


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simFRAP specifications


Unique identifier OMICS_25912
Name simFRAP
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input data A file representing the experiment.
Input format TIFF
Operating system Unix/Linux
Computer skills Advanced
Version 1.0
Stability Stable
Apache Commons, JGoodies libraries
Source code URL
Maintained Yes




No version available


  • person_outline Levi Gheber

Publication for simFRAP

simFRAP citations


Aminopurvalanol A, a Potent, Selective, and Cell Permeable Inhibitor of Cyclins/Cdk Complexes, Causes the Reduction of in Vitro Fertilizing Ability of Boar Spermatozoa, by Negatively Affecting the Capacitation Dependent Actin Polymerization

Front Physiol
PMCID: 5744433
PMID: 29312003
DOI: 10.3389/fphys.2017.01097

[…] of the maximum laser power, 1 scan at 100% laser power for bleaching, and 25 scans for monitoring recovery at 2.4% of the maximum laser power. Recovery curves were recorded and analyzed by using the simFRAP plug-in for Fiji ImageJ ( (Blumenthal et al., ). It computes the diffusion coefficients of fluorescent dye embedded in cell membrane, rega […]


Multi physics interactions drive VEGFR2 relocation on endothelial cells

Sci Rep
PMCID: 5711959
PMID: 29196628
DOI: 10.1038/s41598-017-16786-4
call_split See protocol

[…] inactivated by this treatment and that recovery of fluorescence due to newly synthesized EYFP proteins was not detectable during the period of recovery (10 minutes). Image series were analysed using simFRAP ImageJ plugin ( To calculate diffusion coefficients and using FRAP Caculator ImageJ macro ( […]


A core viral protein binds host nucleosomes to sequester immune danger signals

PMCID: 4950998
PMID: 27362237
DOI: 10.1038/nature18317

[…] nstitutively expresses HMGB1 with a monomeric GFP C-terminal tag. FRAP was carried out using standard methods on a Zeiss LSM 710 confocal microscope. Diffusion coefficients were calculated using the “simFRAP” algorithm (, a simulation based approach to FRAP analysis. […]

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simFRAP institution(s)
The Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer-Sheva, Israel; Department of Biology, Johns Hopkins University, Baltimore, MD, USA
simFRAP funding source(s)
Supported by a grant from the US-Israel Binational Science Foundation, #2009345.

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