simFRAP statistics

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simFRAP specifications

Information


Unique identifier OMICS_25912
Name simFRAP
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input data A file representing the experiment.
Input format TIFF
Operating system Unix/Linux
Computer skills Advanced
Version 1.0
Stability Stable
Requirements
Apache Commons, JGoodies libraries
Source code URL https://www.dropbox.com/s/marzfpic9ed69u4/simFRAP-src.zip?dl=0
Maintained Yes

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Maintainer


  • person_outline Levi Gheber <>

Publication for simFRAP

simFRAP in publications

 (3)
PMCID: 5744433
PMID: 29312003
DOI: 10.3389/fphys.2017.01097

[…] of the maximum laser power, 1 scan at 100% laser power for bleaching, and 25 scans for monitoring recovery at 2.4% of the maximum laser power. recovery curves were recorded and analyzed by using the simfrap plug-in for fiji imagej (https://imagej.nih.gov/ij/plugins/sim-frap/index.html) (blumenthal et al., ). it computes the diffusion coefficients of fluorescent dye embedded in cell membrane, […]

PMCID: 5711959
PMID: 29196628
DOI: 10.1038/s41598-017-16786-4

[…] inactivated by this treatment and that recovery of fluorescence due to newly synthesized eyfp proteins was not detectable during the period of recovery (10 minutes). image series were analysed using simfrap imagej plugin (https://imagej.nih.gov/ij/plugins/sim-frap/index.html). to calculate diffusion coefficients and using frap caculator imagej macro […]

PMCID: 4950998
PMID: 27362237
DOI: 10.1038/nature18317

[…] expresses hmgb1 with a monomeric gfp c-terminal tag. frap was carried out using standard methods on a zeiss lsm 710 confocal microscope. diffusion coefficients were calculated using the “simfrap” algorithm (http://imagej.nih.gov/ij/plugins/sim-frap/index.html), a simulation based approach to frap analysis., statistical details are reported in each figure legend. statistical analyses […]


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simFRAP institution(s)
The Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer-Sheva, Israel; Department of Biology, Johns Hopkins University, Baltimore, MD, USA
simFRAP funding source(s)
Supported by a grant from the US-Israel Binational Science Foundation, #2009345.

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