siRNA Construct Builder specifications

Information


Unique identifier OMICS_27572
Name siRNA Construct Builder
Alternative name Construct Builder
Interface Web user interface
Restrictions to use Academic or non-commercial use
Output data Target siRNA, custom cloning sites, description of the expression vector selected by the user, and the necessary quantity of synthesized oligonucleotides.
Computer skills Basic
Stability Stable
Maintained Yes

Maintainers


  • person_outline Luquan Wang <>
  • person_outline GenScript's siRNA Design Center Team <>

Publication for siRNA Construct Builder

siRNA Construct Builder in publications

 (5)
PMCID: 4159539
PMID: 25175595
DOI: 10.1186/1471-2407-14-639

[…] designated as psisc-upar. the hsp70 sirna vector (psihsp70) was constructed previously in our laboratory []. the mrj sirna insert sequence was designed using sirna designer software and sirna construct builder software (genscript). mrj sirna was designed and constructed into prnat-u6.1/neo using bamhi/hindiii (takara) and designated as psimrj. a negative control for sirna […]

PMCID: 3257043
PMID: 21814176
DOI: 10.1038/ki.2011.250

[…] and performed as directed by technical manual no. 0187 (genscript, piscataway, nj). a small dna insert (roughly 70–80 base pairs, designed husing genscript's sirna target finder and sirna construct builder) encoding a short hairpin rna targeting the gene of interest was cloned into this vector between the mlui and xhoi sites. the sense oligonucleotide sequences of the short […]

PMCID: 3079537
PMID: 21278726
DOI: 10.1038/nature09797

[…] (mountain view, ca). for α5 subunit knockdown studies, two different short hairpin interfering rnas (shrna) directed against the rat chrna5 gene were were designed using the genscript, inc. online construct builder (see for shrna sequence). the shrnas were cloned into the prnat-u6.2/lenti construct containing gfp (genscript, piscataway, nj). control vectors were identical to the expression […]

PMCID: 2928848
PMID: 20711185
DOI: 10.1038/nn.2615

[…] on at least 800 cells by an investigator blinded to the identity of the samples., for mecp2 knockdown, a 70 nucleotide short hairpin interfering rna was designed using the genscript, inc. online construct builder (see for shrna sequence). the shrna was cloned into the prnat-u6.2/lenti virus expression vector from genscript. for bdnf overexpression, the bdnf full-length cdna was purchased […]

PMCID: 2748697
PMID: 19806181
DOI: 10.1371/journal.pbio.1000208

[…] . thus, it is likely that coordinated assembly of lamellipodia and filopodia, controlled by proteins with opposing activity, is essential for growth cone formation and neurite outgrowth., an sirna construct builder program (ambion) was used to generate 55–60-mer oligonucleotides encoding 21-mer hairpin sequences specific to the capzb2 mrna target, with 3′ single-stranded overhangs […]


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siRNA Construct Builder institution(s)
GenScript Corporation, Piscataway, NJ, USA

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