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siRNA Wizard specifications

Information


Unique identifier OMICS_15906
Name siRNA Wizard
Interface Web user interface
Restrictions to use None
Input data A sequence of target gene.
Computer skills Basic
Version 3.1
Stability Stable
Maintained Yes

Maintainer


  • person_outline siRNA Wizard

siRNA Wizard citations

 (16)
library_books

Interleukin 6 Mediates Post Infarct Repair by Cardiac Explant Derived Stem Cells

2017
Theranostics
PMCID: 5706104
PMID: 29187908
DOI: 10.7150/thno.19435

[…] ector system was used to knockdown human IL-6 (pLKO.1-TRC cloning vector, Addgene plasmid #10878). Custom short hairpin oligonucleotides were designed against human IL-6 (shIL-6) using RNAi software (siRNA Wizard v3.1; InvivoGen). A scramble (SCR) construct was created to control for lentiviral transduction. Lentivirus was generated by HEK293 co-transfection followed by filter column concentration […]

call_split

Betanodavirus like particles enter host cells via clathrin mediated endocytosis in a cholesterol , pH and cytoskeleton dependent manner

2017
PMCID: 5299686
PMID: 28179028
DOI: 10.1186/s13567-017-0412-y
call_split See protocol

[…] ccording to the manufacturer’s instructions. siRNA against sbCH (siCH) and sbCL (siCL) were designed based on the full-length mRNA sequences (accession Num: KX989459, KX989460) using the online tools siRNA Wizard v3.1 [] and siDirect V2.0 []. siRNA with an irrelevant sequence to all known genes (siIR) were also designed and the sequences of the sense and antisense siRNA strands were displayed in T […]

library_books

Supernova: A Versatile Vector System for Single Cell Labeling and Gene Function Studies in vivo

2016
Sci Rep
PMCID: 5075795
PMID: 27775045
DOI: 10.1038/srep35747

[…] d the template oligonucleotide for LacZ shRNA scramble control WL091, respectively, using the primers HM082/HM083. The sequence for LacZ shRNA scramble control are designed using internet tool termed siRNA Wizard-Generation of scrambled siRNA sequence. The amplified products were ligated into XhoI/EcoRI sites of pCAG-loxP-stop-loxP-mir30 vector. The shRNA sense and antisense target sequences appea […]

library_books

The increased expression of fatty acid binding protein 9 in prostate cancer and its prognostic significance

2016
Oncotarget
PMCID: 5347732
PMID: 27779102
DOI: 10.18632/oncotarget.12635

[…] lysis after the transient transfection of each of the 3 candidate sequences. The most efficient suppressor sequence was identified from the blot and was used to design short hairpin RNA (shRNA) using siRNA Wizard™ Software(InvivoGen, USA). Two shRNA inserts containing RNAi sequences against FABP9 were cloned into the psiRNA-h7SKGFPzeo plasmid (InvivoGen, USA) separately. PC3-M was stably transfect […]

library_books

LIMT is a novel metastasis inhibiting lncRNA suppressed by EGF and downregulated in aggressive breast cancer

2016
PMCID: 5009810
PMID: 27485121
DOI: 10.15252/emmm.201606198

[…] T (NR_002809) was purchased from BlueHeron and cloned into a pLEX_307 expression vector. As control, eGFP was cloned into pLEX_307. For knockdown of LIMT: Sequences targeting LIMT were designed using siRNA Wizard (http://www.invivogen.com/sirnawizard) and the GPP site (http://www.broadinstitute.org/rnai/public), as well as sequences designed by Dharmacon for siRNAs. Each shRNA sequence was cloned […]

library_books

UBE2B is implicated in myofibrillar protein loss in catabolic C2C12 myotubes

2015
PMCID: 4864198
PMID: 27239408
DOI: 10.1002/jcsm.12060

[…] ex, the primers, and siRNAs were from Sigma‐Aldrich (Saint‐Quentin Fallavier, France). All primers and siRNAs were designed using the PrimerBlast (http://www.ncbi.nlm.nih.gov/tools/primer‐blast/) and siRNA Wizard v3.1 (http://www.sirnawizard.com) software, respectively, and are listed in the Supporting Information section. […]

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