Small non-coding RNA (ncRNA or sRNA) are RNA molecules that do not translate into functional proteins. There are several families of ncRNA, including microRNA, small interfering RNA (siRNA), transfer RNA (tRNA) etc. NcRNA are involved in various biological processes, such as RNA splicing, post-transcriptional modifications, gene silencing, and in diseases including cancer and Alzheimer’s. Small RNA sequencing (sRNA-seq) uses high-throughput method to isolate and sequence small RNA species. Software analysis tools are used for processing, quantification, and identification of sRNA.