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Protocols

SnapDragon specifications

Information


Unique identifier OMICS_04734
Name SnapDragon
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

SnapDragon citations

 (14)
library_books

Implementing the sterile insect technique with RNA interference – a review

2017
PMCID: 5697603
PMID: 29200471
DOI: 10.1111/eea.12575

[…] ort that OTEs are equally probable in conserved and non‐conserved amino acid sequences.It is important to minimize the potential for OTEs by careful design of dsRNAs. ‘E‐RNAi’ (Horn & Boutros, ) and ‘SnapDragon’ (Harvard Medical School, ) are examples of software that automatically design dsRNAs for use with RNAi and search for OTEs in a selection of well‐referenced genomes. If dsRNAs are designed […]

library_books

Investigating the Interplay between Sister Chromatid Cohesion and Homolog Pairing in Drosophila Nuclei

2016
PLoS Genet
PMCID: 4991795
PMID: 27541002
DOI: 10.1371/journal.pgen.1006169

[…] m Qiagen, with a GFP-expressing plasmid as a co-transfection marker. When using Effectene, the amount of dsRNA was reduced to 1.2 μg per well in a 24-well plate. dsRNA primers were designed using the SnapDragon tool for primer design (http://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl) and synthesized by PCR amplification from genomic DNA followed by an in vitro transcription reaction using a MEG […]

call_split

Keap1 Independent Regulation of Nrf2 Activity by Protein Acetylation and a BET Bromodomain Protein

2016
PLoS Genet
PMCID: 4883770
PMID: 27233051
DOI: 10.1371/journal.pgen.1006072
call_split See protocol

[…] ere used to knock down Fs(1)h, CncC, Keap1 whereas Fs(1)h-L was knocked down using the dsRNA described by Kockmann et al. []. dsRNA targeting the unique 3’ UTR region of Fs(1)h-S was designed by the ‘SnapDragon’ webservice. The sequences of primers used to generate amplicons for dsRNA synthesis are provided in Table B in . […]

library_books

Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells

2015
PMCID: 4555228
PMID: 26199285
DOI: 10.1534/g3.115.019364

[…] We used UP-TORR (http://flyrnai.org/up-torr) () to identify appropriate reagent templates in our collection. As needed for two reagents per gene coverage, we designed additional dsRNAs using SnapDragon (http://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl). We used standard protocols to prepare dsRNAs (; see http://www.flyrnai.org/DRSC-PRR.html for protocol). In brief, we used liquid handl […]

library_books

MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth

2015
PLoS Genet
PMCID: 4395465
PMID: 25875245
DOI: 10.1371/journal.pgen.1005124

[…] end to generate dsRNA targeting PxmALP (GenBank accession no. KC841472) and mALP1 from H. armigera (GenBank accession no. EU729322.1), or EGFP (GenBank accession no. KC896843) were designed using the SnapDragon tool (http://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl). Primers to generate dsRNA to PxABCC2 (GenBank accession no. KM245561) and PxABCC3 (GenBank accession no. KM245562) were designed […]

library_books

Cloning, Characterization and Effect of TmPGRP LE Gene Silencing on Survival of Tenebrio Molitor against Listeria monocytogenes Infection

2013
Int J Mol Sci
PMCID: 3856074
PMID: 24240808
DOI: 10.3390/ijms141122462

[…] was used to generate a template for in vitro transcription using gene-specific primers tailed with a T7 promoter sequence (). To minimize potential off-target effects, primers were designed using the SnapDragon dsRNA design software (http://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl) [], which detected no potential off-target regions in the sequence [,]. The dsRNA was prepared with the Ampliscri […]


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