A fast and easy desktop GUI tool for the identification of genomic variants from pooled sequencing and individual sequencing data. Using SNVerGUI, users can perform sophisticated variant detection by simply configuring several parameters in a friendly graphical user interface. Compared with other methods for variant calling, our approach is unique in that it is applicable to both individual and pooled sequencing data. SNVerGUI supports commonly used input and output file formats that allows SNVerGUI to be seamlessly integrated into common NGS data analysis pipelines.