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SolexaQA | At-a-glance quality assessment of Illumina second-generation sequencing data

A user-friendly software package designed to generate detailed statistics and at-a-glance graphics of sequence data quality both quickly and in an automated fashion. SolexaQA contains associated software to trim sequences dynamically using the quality scores of bases within individual reads. It produces standardized outputs within minutes, thus facilitating ready comparison between flow cell lanes and machine runs, as well as providing immediate diagnostic information to guide the manipulation of sequence data for downstream analyses.

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SolexaQA forum

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SolexaQA classification

SolexaQA specifications

Unique identifier:
Command line interface
Input format:
Biological technology:
Illumina, Life Technologies, Roche
Programming languages:
Perl, R
Computer skills:
Matrix2png, GD graphics
Software type:
Restrictions to use:
Output format:
FASTQ, PDF, text
Operating system:
Unix/Linux, Mac OS, Windows
GNU General Public License version 2.0


  • DynamicTrim

SolexaQA distribution


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SolexaQA support


  • Murray Cox <>


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Institute of Molecular BioSciences, Massey University, New Zealand; The Allan Wilson Centre for Molecular Ecology and Evolution, New Zealand; The Bio-Protection Research Centre, New Zealand; Institute of Veterinary, Animal and Biomedical Sciences, Massey University, New Zealand; Massey Genome Service, Massey University New Zealand

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