SPADS specifications


Unique identifier OMICS_24505
Alternative names Specific Primers and Amplicon Design Software, GENOPLANTETMS.P.A.D.S.
Interface Web user interface
Restrictions to use Academic or non-commercial use
Input data A sequence and a localisation.
Input format (FASTA,GenBank)+ GenBank
Computer skills Basic
Version 1.2.0
Stability Stable
Maintained Yes


  • Plants and Fungi
    • Arabidopsis thaliana
    • Saccharomyces cerevisiae


  • person_outline Pierre Rouze
  • person_outline SPADS Team

Publication for Specific Primers and Amplicon Design Software

SPADS citations


Genetic diversity, linkage disequilibrium and power of a large grapevine (Vitis vinifera L) diversity panel newly designed for association studies

BMC Plant Biol
PMCID: 4802926
PMID: 27005772
DOI: 10.1186/s12870-016-0754-z

[…] 9 and 12 [] and berry weight on chromosome 17 []. Primer pairs were automatically designed in exons [] to amplify one specific amplicon of 400–1400 bp per gene, using an automated pipeline combining SPADS v1.0 [] and PRIMER3 v2.3.6 [] softwares (detailed procedure available upon request). Within each genomic region, 55–60 amplicons were selected to optimize sequencing (longest possible exon in on […]


Transcript profiling of cytokinin action in Arabidopsis roots and shoots discovers largely similar but also organ specific responses

BMC Plant Biol
PMCID: 3519560
PMID: 22824128
DOI: 10.1186/1471-2229-12-112

[…] osystems), controlled and evaluated by the 7500 Software V2.0.1 RC. In order to exclude primers binding to unspecific sequences, they were designed using GENOPLANTE™S.P.A.D.S ( [] or NCBI Primer-BLAST ( []. The primer sequences are listed in Additional file : Table S7. […]

SPADS institution(s)
Laboratoire Associé de l’Institut National de Recherche Agronomique, France; Department of Plant Systems Biology, Flanders Interuniversity Institute of Biotechnology, Ghent University, Gent, Belgium; Unité de Recherche en Génomique Végétale, UMR INRA-CNRS, Evry, France
SPADS funding source(s)
Supported by the French GENOPLANTE™ program.

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