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Protocols

SparkMaster specifications

Information


Unique identifier OMICS_19440
Name SparkMaster
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input data An individual image.
Output data A data table showing the analysis of individual sparks, general analysis, and information about the analyzed image; and the outputted image.
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
Computer skills Medium
Stability Stable
Requirements
ImageJ
Maintained Yes

Versioning


No version available

Publication for SparkMaster

SparkMaster citations

 (30)
library_books

Effects of rogue ryanodine receptors on Ca2+ sparks in cardiac myocytes

2018
R Soc Open Sci
PMCID: 5830753
PMID: 29515864
DOI: 10.1098/rsos.171462

[…] used for line-scan images at a sample rate of 512 frames s−1. The samples were excited at 488 nm. All line-scan measurements were performed at room temperature (23–25°C). Images were processed by the SparkMaster software []. […]

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Structural heterogeneity of the rat pulmonary vein myocardium: consequences on intracellular calcium dynamics and arrhythmogenic potential

2018
Sci Rep
PMCID: 5818479
PMID: 29459735
DOI: 10.1038/s41598-018-21671-9
call_split See protocol

[…] um of the derivative signal was considered as the maximum rise time. A pseudo colour LUT was then applied to each pixel in function of its maximum rise time.Sparks linescan images were analysed using SparkMaster plugin for ImageJ software. The initial parameters were 3.8 for criteria and 10 fluorescence units for background. Spark frequency, amplitude, full duration at half maximum (FDHM), full wi […]

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Simvastatin activates single skeletal RyR1 channels but exerts more complex regulation of the cardiac RyR2 isoform

2018
Br J Pharmacol
PMCID: 5825303
PMID: 29278865
DOI: 10.1111/bph.14136
call_split See protocol

[…] apphire laser, and the fluorescence emitted was measured at >515 nm. Images were acquired in line scan mode (every 6 ms). Ca2+ sparks were identified and analysed with ImageJ software (NIH) using the Sparkmaster plugin (Picht et al., ). Events with a full width at half maximum <1 μm were filtered from the data. […]

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Angiotensin II Overstimulation Leads to an Increased Susceptibility to Dilated Cardiomyopathy and Higher Mortality in Female Mice

2018
Sci Rep
PMCID: 5772611
PMID: 29343862
DOI: 10.1038/s41598-018-19436-5
call_split See protocol

[…] and Ca2+ sparks on quiescent ventricular myocytes were acquired using a Zeiss LSM 710 laser scanning microscope in line scan mode. Ca2+ sparks parameters were measured and quantified using the ImageJ Sparkmaster plugin. […]

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Hierarchical statistical techniques are necessary to draw reliable conclusions from analysis of isolated cardiomyocyte studies

2017
PMCID: 5852514
PMID: 29016722
DOI: 10.1093/cvr/cvx151
call_split See protocol

[…] ing 2 mM Ca2+. The control group consisted of myocytes isolated from age-matched controls. Data were viewed using Ionwizard and ImageJ (NIH) and analysed with a combination of custom-built macros and Sparkmaster. Amplitude and morphology of five Ca2+ transients per cell were averaged to give final result for that cell. Sparks were treated as separate data points. […]

library_books

Epac2 Rap1 Signaling Regulates Reactive Oxygen Species Production and Susceptibility to Cardiac Arrhythmias

2017
PMCID: 5510674
PMID: 27649969
DOI: 10.1089/ars.2015.6485

[…] n. Cells were only used if they responded consistently to each electrical stimulation and showed no signs of spontaneous activity under control conditions.Image analysis was done using ImageJ and the Sparkmaster plug-in, which allows automatic detection of Ca2+ sparks. Ca2+ imaging data are presented as the mean ± SEM and statistical significance was assessed using a t-test, chi-squared test, or A […]

Citations

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SparkMaster institution(s)
Department of Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA
SparkMaster funding source(s)
Supported by National Heart, Lung, and Blood Institute Grants HL-30077, HL-80101, and HL-062231 and by Grants from the American Heart Association (0550170Z and 0530309Z).

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