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Spectrum Mill specifications


Unique identifier OMICS_14298
Name Spectrum Mill
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input format FASTA
Biological technology Agilent Technologies
Operating system Windows
Programming languages Java, Perl
License Commercial
Computer skills Medium
Version 05.00
Stability Stable
IIS, MSFileReader
Maintained Yes


No version available


  • person_outline Spectrum Mill Team

Spectrum Mill citations


Specific protein carbonylation in human breast cancer tissue compared to adjacent healthy epithelial tissue

PLoS One
PMCID: 5875748
PMID: 29596499
DOI: 10.1371/journal.pone.0194164

[…] to liquid chromatography-tandem mass spectrometry (lc-ms/ms) analysis using q-tof lc/ms (agilent technologies, santa clara, ca). peptide search and protein identification were performed using the spectrum mill ms proteomic workbench (agilent technologies, santa clara, ca). trypsin-digested bovine serum albumin (bsa) was used as a quality control for ms analysis., two-color western blot […]


First evidence for a multienzyme complex of lipid biosynthesis pathway enzymes in Cunninghamella bainieri

Sci Rep
PMCID: 5814418
PMID: 29449592
DOI: 10.1038/s41598-018-21452-4

[…] 300 until 3000 m/z. auto ms/ms mode applied from 50 until 3000 m/z with total maximum precursor of 4 in a cycle and exception of 2 spectra for 1 minute. identification of peptides was performed by spectrum mill ms proteomics workbench (rev b.04.00.127; agilent technologies). cysteine carbamidomethylation was set as fix and variable modification. search was done online for mucorales […]


Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non alcoholic fatty liver disease

Proteome Sci
PMCID: 5813374
PMID: 29456458
DOI: 10.1186/s12953-018-0131-y

[…] using agilent masshunter data acquisition software (b.03.00)., tandem mass spectra were extracted and charge state deconvoluted by mass hunter version b.03.00 (agilent technologies, usa), while spectrum mill and x!tandem (the gpm, thegpm.org; version 2007.01.01.1) were used for protein identification. deisotoping was not performed. both spectrum mill and x!tandem were set up to search […]


Quantitative mass spectrometry analysis reveals a panel of nine proteins as diagnostic markers for colon adenocarcinomas

PMCID: 5862596
PMID: 29568375
DOI: 10.18632/oncotarget.24418

[…] (n = 11) labelled with the itraq label 117. the itraq labelled samples were pooled and subjected to off-gel fractionation followed by lc-ms/ms. the lc-ms/ms data was analyzed independently by spectrum mill (sm). 514, 354, 569 and 581 proteins were identified with 2 or more unique peptides in set 1, set 2, set 3 and set 4 respectively, of the itraq labelled samples from sm analysis. […]


Differential abundance and transcription of 14 3 3 proteins during vegetative growth and sexual reproduction in budding yeast

Sci Rep
PMCID: 5794856
PMID: 29391437
DOI: 10.1038/s41598-018-20284-6

[…] labelled with 114, 116 and peptides from metaphase-i arrested cells with 115 and 117 labels. saccharomyces cerevisiae was set as taxonomy during analysis of data. data analysis was performed using spectrum mill (from agilent) as described previously., a required number of cells were taken in the fresh tube and washed twice with pbs ph 7.2. cells were resuspended in 70% ethanol for 1 min […]


New Interactors of the Truncated EBNA LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells

PMCID: 5789362
PMID: 29303964
DOI: 10.3390/cancers10010012

[…] peptides were separated for 30 min on a gradient of 5 to 90% acetonitrile. the fragmentation voltage was 1.3 v. the resulting ms/ms spectra were searched against a human protein database using the spectrum mill rev. a 03 03 84 sr4 software package (agilent). to quantify a particular peptide in the sample, the ion trap was set in mrm (multiple reaction monitoring) mode., the tebna-lp partners […]

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