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- person_outline John Weinstein
Publication for SpliceCenter
Valid gene expression normalization by RT qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis
[…] constructed primers was corroborated by PrimerBLAST and cross-checked using the UCSC Genome Browser (University of California, CA, USA) (Supplementary Data ). Using NCBI PrimerBLAST and PrimerCheck (SpliceCenter of Genomics and Bioinformatics Group, LMP, CCR, NCI), we could confirm that primers also targeted possible splicing and transcript variants (except for RNA18S5), whereas no pseudogenes, r […]
Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
[…] ence similarity of the highly homologous DC-SIGN and L-SIGN transcripts, primers were designed in regions common to the splice variants but unique to the respective transcripts with the assistance of SpliceCenter software (In Silico Solutions, Falls Church, VA). Major bands were found at 707 bp (DC-SIGN), and 229 bp (L-SIGN), and 166 bp (L-SIGN), corresponding to RefSeqs DC-SIGN (CD209) transcript […]
Alternative Splicing for Diseases, Cancers, Drugs, and Databases
[…] s from Affymetrix probe set inputs. ASTALAVISTA  provides alternative splicing prediction for transcriptome data from GENCODE, REFSEQ, and ENSEMBL as well as from custom gene datasets. Furthermore, SpliceCenter  is a web server for predicting the influence of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based data.Both PolyA_DB  and AltTrans provide the information for alter […]
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