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SpliceCenter specifications


Unique identifier OMICS_18087
Name SpliceCenter
Interface Web user interface
Restrictions to use None
Output format TXT
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline John Weinstein

Publication for SpliceCenter

SpliceCenter citations


Valid gene expression normalization by RT qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis

Sci Rep
PMCID: 5677027
PMID: 29116140
DOI: 10.1038/s41598-017-15281-0

[…] constructed primers was corroborated by PrimerBLAST and cross-checked using the UCSC Genome Browser (University of California, CA, USA) (Supplementary Data ). Using NCBI PrimerBLAST and PrimerCheck (SpliceCenter of Genomics and Bioinformatics Group, LMP, CCR, NCI), we could confirm that primers also targeted possible splicing and transcript variants (except for RNA18S5), whereas no pseudogenes, r […]


Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication

Sci Rep
PMCID: 5269613
PMID: 28128342
DOI: 10.1038/srep41389
call_split See protocol

[…] ence similarity of the highly homologous DC-SIGN and L-SIGN transcripts, primers were designed in regions common to the splice variants but unique to the respective transcripts with the assistance of SpliceCenter software (In Silico Solutions, Falls Church, VA). Major bands were found at 707 bp (DC-SIGN), and 229 bp (L-SIGN), and 166 bp (L-SIGN), corresponding to RefSeqs DC-SIGN (CD209) transcript […]


Alternative Splicing for Diseases, Cancers, Drugs, and Databases

Sci World J
PMCID: 3674688
PMID: 23766705
DOI: 10.1155/2013/703568

[…] s from Affymetrix probe set inputs. ASTALAVISTA [] provides alternative splicing prediction for transcriptome data from GENCODE, REFSEQ, and ENSEMBL as well as from custom gene datasets. Furthermore, SpliceCenter [] is a web server for predicting the influence of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based data.Both PolyA_DB [] and AltTrans provide the information for alter […]


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SpliceCenter institution(s)
Genomics & Bioinformatics Group, Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Tiger Team Consulting, Fairfax, VA, USA; Gene Silencing Section, Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Department of Biostatistics, Bioinformatics, and Biomathematics, Georgetown University Medical Center, Washington, DC, USA; Department of Bioinformatics and Computational Biology, M. D. Anderson Cancer Center, Houston, TX, USA
SpliceCenter funding source(s)
Supported in part by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research and in part by Tiger Team Consulting.

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