Allows investigation the impact of splice variation on the interpretation of data derived from those technologies. SpliceCenter provides analysis of RT-PCR primer/probe sets, effectors of RNAi, microarrays, and protein-targeting technologies. It can analyze the impact of alternative splicing on studies of transcripts and proteins. The tool is composed of four modules: Primer-Check, Array-Check, siRNA-Check, and PeptideCheck. It is able to identify the target locations of oligonucleotides or peptides and returns results on a graphical form with clickable hyperlinks.
Genomics & Bioinformatics Group, Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Tiger Team Consulting, Fairfax, VA, USA; Gene Silencing Section, Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA; Department of Biostatistics, Bioinformatics, and Biomathematics, Georgetown University Medical Center, Washington, DC, USA; Department of Bioinformatics and Computational Biology, M. D. Anderson Cancer Center, Houston, TX, USA
SpliceCenter funding source(s)
Supported in part by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research and in part by Tiger Team Consulting.