splitter specifications


Unique identifier OMICS_16156
Software type Package/Module
Interface Command line interface
Restrictions to use None
Input data Reads one or more nucleotide or protein sequences. The input is a standard EMBOSS sequence query (also known as a 'USA').
Output data A standard EMBOSS sequence file.
Operating system Unix/Linux, Mac OS, Windows
Programming languages C
License GNU General Public License version 3.0
Computer skills Advanced
Stability Stable
Maintained Yes


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  • person_outline Gary Williams <>

splitter citations

PMCID: 4725681

[…] were in accordance with the manufacturers’ instructions., the original reads files were analysed by coral, an error correction algorithm [36]. the output (fasta format) was split using fastx_barcode_splitter (from the fastx-toolkit [37]) to isolate the reads from the different samples tested in the same run using the mid sequences. prior to clustering, the primer sequences were trimmed using […]

PMCID: 4163659

[…] prepared and sequenced. raw sequencing reads were filtered by illumina hiseq control software to generate pass-filter (pf) reads for further analysis., pf reads were de-multiplexed using the barcode splitter in fastx-toolkit and the reads from each library were mapped to mouse genome build mm10 with refseq gene annotation, using tophat 2 software, allowing only unique alignment for each read. […]

splitter institution(s)
MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

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