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Spotlite specifications


Unique identifier OMICS_16241
Name Spotlite
Interface Web user interface
Restrictions to use None
Input data A tab-delimited file with experiment ID, experiment type, bait, prey and spectral count columns
Output data Protein interaction network with a score and a visualization
Computer skills Basic
Stability Stable
Maintained Yes



  • person_outline Michael Major

Publication for Spotlite

Spotlite citations


The Aspergillus flavus Homeobox Gene, hbx1, Is Required for Development and Aflatoxin Production

PMCID: 5666362
PMID: 29023405
DOI: 10.3390/toxins9100315

[…] he surface of the plate then transferred to a sterile 15 mL Sarstedt tube. When necessary, dilutions of conidial suspensions were made in 0.01% Triton X-100 and conidia counted using a hemocytometer (SPotlite hemocytometer, McGaw Park, IL, USA). For determination of sclerotial numbers, a single 6 mm plug was removed from a point on the colony that was half the distance from the center to the edge […]


A neomorphic cancer cell specific role of MAGE A4 in trans lesion synthesis

Nat Commun
PMCID: 4935975
PMID: 27377895
DOI: 10.1038/ncomms12105

[…] lity cutoffs resulting in a 1% false discovery rate were selected individually for each experiment. The resulting spectral count data from controls and HA–RAD18-WT APMS experiment were input into the Spotlite web application using SAINTexpress (version 3.1.0), to determine protein–protein interaction probabilities by modelling the expected spectral count distribution of true and false interactions […]


Bone Mineral Density in Thalassemia Major Patients from Antalya, Turkey

PMCID: 3388304
PMID: 22778734
DOI: 10.1155/2012/573298

[…] reatine, and other parameters were measured using an Olympus AU400 autoanalyzer and reagents (USA). The concentration of calcium ions (Ca2+) was measured by means of a Medica Prolyte Menarini Il-Lyte Spotlite Electrolyte Analyzer System. Serum magnesium (Mg2+) was measured in a Boehringer/Hitachi spectrometer using commercially available kits from Roche Diagnostics, Basel, Switzerland. The serum p […]


Development and Implementation of a High Throughput Screen for the Human Sperm Specific Isoform of Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDHS)

PMCID: 3134944
PMID: 21760877
DOI: 10.2174/1875397301105010030

[…] plates of 0.88 and CVs <5% (Fig. ) demonstrating a suitable assay window and acceptable variability for HTS as typically defined [,,] and [NIH Chemical Genomics Center Assay Guidance Manual: (]. For HTS, typically 10-20 compound plates are screened in one run. To assess the stability of the assay over such a ten 384-well plate run, a varia […]


Straw blood cell count, growth, inhibition and comparison to apoptotic bodies

BMC Cell Biol
PMCID: 2397387
PMID: 18492269
DOI: 10.1186/1471-2121-9-26

[…] Determining SBC used both an in-solution and an in-air method. In solution, a Hemacytometer (SPotlite, Baxter Healthcare Corporation) was used to determine SBC straw cells; visible dark dots with diameters around 1 μm were counted and five random squares were used to average the counts. In th […]


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Spotlite institution(s)
The Brody School of Medicine at East Carolina University, Greenville, CN, USA
Spotlite funding source(s)
This work was supported by the Sidney Kimmel Foundation for Cancer Research.

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