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Spotlite specifications

Information


Unique identifier OMICS_16241
Name Spotlite
Interface Web user interface
Restrictions to use None
Input data A tab-delimited file with experiment ID, experiment type, bait, prey and spectral count columns
Output data Protein interaction network with a score and a visualization
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Maintainer


  • person_outline Michael Major <>

Publication for Spotlite

Spotlite in publications

 (7)
PMCID: 5666362
PMID: 29023405
DOI: 10.3390/toxins9100315

[…] surface of the plate then transferred to a sterile 15 ml sarstedt tube. when necessary, dilutions of conidial suspensions were made in 0.01% triton x-100 and conidia counted using a hemocytometer (spotlite hemocytometer, mcgaw park, il, usa). for determination of sclerotial numbers, a single 6 mm plug was removed from a point on the colony that was half the distance from the center to the edge […]

PMCID: 4935975
PMID: 27377895
DOI: 10.1038/ncomms12105

[…] cutoffs resulting in a 1% false discovery rate were selected individually for each experiment. the resulting spectral count data from controls and ha–rad18-wt apms experiment were input into the spotlite web application using saintexpress (version 3.1.0), to determine protein–protein interaction probabilities by modelling the expected spectral count distribution of true and false […]

PMCID: 3388304
PMID: 22778734
DOI: 10.1155/2012/573298

[…] and other parameters were measured using an olympus au400 autoanalyzer and reagents (usa). the concentration of calcium ions (ca2+) was measured by means of a medica prolyte menarini il-lyte spotlite electrolyte analyzer system. serum magnesium (mg2+) was measured in a boehringer/hitachi spectrometer using commercially available kits from roche diagnostics, basel, switzerland. , […]

PMCID: 3134944
PMID: 21760877
DOI: 10.2174/1875397301105010030

[…] of 0.88 and cvs <5% (fig. ) demonstrating a suitable assay window and acceptable variability for hts as typically defined [,,] and [nih chemical genomics center assay guidance manual: (http://spotlite.nih.gov/assay/index.php/table_of_contents)]. for hts, typically 10-20 compound plates are screened in one run. to assess the stability of the assay over such a ten 384-well plate run, […]

PMCID: 2852845
PMID: 20386645
DOI: 10.3390/ijms11020386

[…] multidimensional information content. it may also serve as a tool to compare different surface interaction models []., this study was supported from the european union 6th framework programme (spotlite), the swedish research council (vr-621-2006-3197), and the knut and alice wallenberg foundation. personal scholarship awarded by leif jonsson’s minnesfond to h. b. and wenner-gren foundation […]


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Spotlite institution(s)
The Brody School of Medicine at East Carolina University, Greenville, CN, USA
Spotlite funding source(s)
This work was supported by the Sidney Kimmel Foundation for Cancer Research.

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