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SpotTracker specifications


Unique identifier OMICS_06581
Name SpotTracker
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
Computer skills Medium
Stability Stable
Maintained Yes


No version available


  • person_outline Daniel Sage <>

Publication for SpotTracker

SpotTracker citations


Quantitative analysis of circadian single cell oscillations in response to temperature

PMCID: 5749732
PMID: 29293562
DOI: 10.1371/journal.pone.0190004

[…] values. outliers were removed from images using the built-in -fiji option with the settings "rad 1" and "threshold 100". for fibroblast movies, single cell bioluminescence was traced using the spottracker plugin [], which enabled us to accurately track cells that move and oscillate at the same time. we then calculated the signal strength as the mean over a small number of pixels […]


Thermal gradient induced tweezers for the manipulation of particles and cells

PMCID: 5113121
PMID: 27853191
DOI: 10.1038/srep35814

[…] of single particle. we recorded the time-trajectory of a trapped particle over a time interval of 21 seconds (the duration is only limited by the video capture and image analysis software) using spottracker algorithm together with imagej software. our experimental results are shown in . as seen from , the trajectory of a single particle trapped by meth is typically within micrometer scale. […]


Non cell autonomous cues for enhanced functionality of human embryonic stem cell derived cardiomyocytes via maturation of sarcolemmal and mitochondrial KATP channels

PMCID: 5039730
PMID: 27677332
DOI: 10.1038/srep34154

[…] that were uniformly anchored to the tips of the cantilevers were included in the analysis. the displacement of fluorescent microbeads at the top of the cantilevers was then tracked with using the spottracker plug-in in imagej (national institutes of health). microtissues were treated with the thyroid hormone triiodothyronine (t3, 100 nm) at the time of seeding for 6 consecutive days. […]


The myosin X motor is optimized for movement on actin bundles

PMCID: 5025751
PMID: 27580874
DOI: 10.1038/ncomms12456

[…] the assay was carried out at 30 °c., to analyse the data, individual trajectories were extracted from image sequences by means of imagej (nih, bethesda, md) and imagej plugins: particletracker and spottracker. image sequences were pre-filtered with a log filter (mexicanhat) as described to improve signal-to-noise ratio before tracking trajectories. a step-fitting algorithm implemented […]


The molecular mechanism of photochemical internalization of cell penetrating peptide cargo photosensitizer conjugates

PMCID: 4685267
PMID: 26686907
DOI: 10.1038/srep18577

[…] images were obtained every 5 s. for measuring the fluorescence intensities at endosomal dots in the time-lapse images, imagej software (national institutes of health, bethesda, md) equipped with the spottracker 2d plug-in was used., how to cite this article: ohtsuki, t. et al. the molecular mechanism of photochemical internalization of cell penetrating peptide-cargo-photosensitizer conjugates. […]


Myosin binding protein C corrects an intrinsic inhomogeneity in cardiac excitation contraction coupling

PMCID: 4380226
PMID: 25839057
DOI: 10.1126/sciadv.1400205

[…] in a random order. thin filament motility on thick filaments was recorded (120 frames/s, 1000 frames) at each pca in multiple areas on the flow cell. the image stacks were processed using imagej spottracker 2d with spatial resolution being 30 nm as reported (). the x- and y-positions of linear tracks less than 800 nm in length were recorded in excel (microsoft). the instantaneous velocity […]

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SpotTracker institution(s)
Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

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