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SprayQc specifications

Information


Unique identifier OMICS_08216
Name SprayQc
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

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Publication for SprayQc

SprayQc in publications

 (11)
PMCID: 5795394
PMID: 29208753
DOI: 10.1074/mcp.RA117.000315

[…] 95% buffer b and re-equilibrated with buffer a. column temperature was kept at 50 °c by an in-house designed oven with a peltier element and operational parameters were monitored in real time by the sprayqc software. ms data were acquired with a top15 shotgun proteomics method, where in each cycle a full scan is followed by up to 15 data-dependent ms/ms scans. target value for the full scan ms […]

PMCID: 5714591
PMID: 29166617
DOI: 10.1016/j.celrep.2017.10.097

[…] 1.9 μm resin (dr. maisch, ammerbuch, germany). column temperature was kept at 50°c by an in-house-designed oven with a peltier element, and operational parameters were monitored in real time by the sprayqc software (). peptides were loaded with buffer a (0.1% [v/v] formic acid) and eluted with a nonlinear gradient of 5%–60% buffer b (0.1% [v/v] formic acid, 80% [v/v] acetonitrile) at a flow […]

PMCID: 5383787
PMID: 28126900
DOI: 10.1074/mcp.O116.065136

[…] buffer b. the flow rate was kept at 350 nl/min., column temperature was kept at 60 °c by an in-house-developed oven containing a peltier element, and parameters were monitored in real time by the sprayqc software ()., ms data was acquired with the thermo xcalibur software version 3.0.63, a topn method where n could be up to 100. this method in principle allows a very large number of precursor […]

PMCID: 5199119
PMID: 28007936
DOI: 10.15252/msb.20167357

[…] wash of 98% buffer b at a flow rate of 350 nl/min. column temperature was kept at 60°c by a peltier element containing in‐house‐developed oven, and parameters were monitored in real time by the sprayqc software (scheltema & mann, ). ms data were acquired with a top15 data‐dependent ms/ms scan method (topn method). target values for the full‐scan ms spectra were 3 × 106 charges […]

PMCID: 5075284
PMID: 27745970
DOI: 10.1016/j.cell.2016.09.031

[…] a non-linear 270 min gradient of 5%–60% buffer b (80% acn, 0.5% formic acid) at a flow rate of 250 nl/min and a column temperature of 50°c. operational parameters were real-time monitored by the sprayqc software (). the q exactive was operated in a data dependent mode with a survey scan range of 300-1750 m/z and a resolution of 70’000 at m/z 200. up to 5 most abundant isotope patterns […]


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SprayQc institution(s)
Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany

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