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Protocols

SR-Tesseler specifications

Information


Unique identifier OMICS_09934
Name SR-Tesseler
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use None
Operating system Windows
Programming languages C++
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Maintainer


  • person_outline Florian Levet

Publication for SR-Tesseler

SR-Tesseler citations

 (9)
library_books

Ca2+ signals initiate at immobile IP3 receptors adjacent to ER plasma membrane junctions

2017
Nat Commun
PMCID: 5686115
PMID: 29138405
DOI: 10.1038/s41467-017-01644-8

[…] crospheres (0.1 µm diameter, ThermoFisher) were used to verify that there was no significant drift during image acquisition.Analysis of the distribution of fluorophores within puncta used the program SR-Tesseler, which effectively encloses every blinking event ( = localization) within a field (Voronoi tessellation), the boundaries of which are drawn to be equidistant between each localization and […]

call_split

Cortical actin contributes to spatial organization of ER–PM junctions

2017
Mol Biol Cell
PMCID: 5687020
PMID: 28954864
DOI: 10.1091/mbc.E17-06-0377
call_split See protocol

[…] ion precision greater than 20 nm, or 3) identified as repetitive detections due to multiple blinking. Also, to achieve better consistency when performing Ripley’s K analysis and image segmentation by SR-Tesseler, a region of interest (ROI) covered by the cell was cropped from each localization data set. Ripley’s K analysis was performed within the platform, which implements an algorithm optimizati […]

library_books

Strength of Neisseria meningitidis binding to endothelial cells requires highly ordered CD147/β2 adrenoceptor clusters assembled by alpha actinin 4

2017
Nat Commun
PMCID: 5461506
PMID: 28569760
DOI: 10.1038/ncomms15764

[…] ixels set to 50. 2D and 3D reconstructions were obtained from stacks (16 planes) with 50 nm axial step size, using the LAS X Software (Leica). The complex organization of CD147 was analysed using the SR-Tesseler Software using default parameters with a density factor=1.3 (ref. ). Quantifications were performed on seven 2D-reconstruction images (mean cluster size=37,700±2,715 nm2). Quantification o […]

library_books

Determining the bacterial cell biology of Planctomycetes

2017
Nat Commun
PMCID: 5394234
PMID: 28393831
DOI: 10.1038/ncomms14853

[…] ith Nile Red and DAPI.For dSTORM experiments a 0.01% Polylysine coated glass bottom dish (MatTek) with Alexa 647 labelled dextran at a final concentration of 5 μg ml−1 served as negative control. The SR-Tesseler method was used for cluster analysis in control and P. limnophila/G. obscuriglobus feeding experiments employing a minimum range of 40 nm and a minimum localization of 20 as previously des […]

library_books

Structural basis for plant plasma membrane protein dynamics and organization into functional nanodomains

2017
eLife
PMCID: 5536944
PMID: 28758890
DOI: 10.7554/eLife.26404.022

[…] llowing long acquisition in oblique illumination mode, and a sensitive Evolve EMCCD camera (Photometrics, Tucson, USA), see . Images acquisitions and processing were done as previously described by ).SR-Tesseler software was used to produce voronoï diagrams, and subsequently quantify molecule organization parameters as previously recommended (). Taking in account fluorophore photophysical paramete […]

library_books

Molecular signatures of mu opioid receptor and somatostatin receptor 2 in pancreatic cancer

2016
Mol Biol Cell
PMCID: 5221597
PMID: 27682590
DOI: 10.1091/mbc.E16-06-0427

[…] s, error bars represent SEM calculated individually for each data point. All presented images were color inverted for clarity. No other modifications were done. Image tessellation was performed using SR-Tesseler software as previously described (). Brief details are provided in the Supplemental Methods. […]

Citations

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SR-Tesseler institution(s)
Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France

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