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SSAKE

Provides a de novo assembler for short DNA sequence reads. SSAKE is designed to help leverage the information from short sequences reads by assembling them into contigs and scaffolds that can be used to characterize novel sequencing targets. SSAKE assembles whole reads (not k-mers) and as such, is well-suited for structural variant assembly/detection. SSAKE is written in PERL and runs on Linux. SSAKE cycles through short sequence reads stored in a hash table and progressively searches through a prefix tree for extension candidates. The algorithm assembled 25 to 300 bp (genome, transcriptome, amplicon) reads from viral, bacterial and fungal genomes. SSAKE is lightweight, simple to setup & run and robust.

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1 user review

1 user review

Nick's avatar image

Nick

Ssake is super easy to use and produces accurate results.
It is lightweight and has no dependencies, I highly recommend it. Because it uses coverage info, assemblies are stratified which makes it also suitable for transcriptome and meta genome assemblies. It is not as greedy as most people report, the correct path is chosen no based on longest overlap, but coverage and reads having a matching pairing partner already assembled

SSAKE forum

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SSAKE versioning

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No versioning.

SSAKE classification

SSAKE specifications

Software type:
Package/Module
Restrictions to use:
None
Computer skills:
Advanced
Maintained:
Yes
Debian:
https://tracker.debian.org/pkg/ssake
Interface:
Command line interface
Operating system:
Unix/Linux
Stability:
Stable
Galaxy:
https://toolshed.g2.bx.psu.edu/repository?repository_id=c61522c3ccd9a473

SSAKE support

Maintainer

  • Rene Warren < >

Credits

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Publications

Institution(s)

British Columbia Cancer Agency, Genome Sciences Centre, Vancouver, Canada; J. Craig Venter Institute, Rockville, MD, USA

Link to literature

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