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A package for error-correcting DNA barcodes. Hamming allows one run of a massively parallel pyrosequencer to process up to 1544 samples simultaneously. The tagged barcoding strategy can be used to obtain sequences from hundreds of samples in a single sequencing run, and to perform phylogenetic analyses of microbial communities from pyrosequencing data. The combination of error-correcting barcodes and massively parallel sequencing rapidly revolutionizes our understanding of microbial habitats located throughout our biosphere, as well as those associated with our human bodies.

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Standalone hamming classification

Standalone hamming specifications

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Standalone hamming distribution


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Standalone hamming support



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Department of Computer Science, University of Colorado, Boulder, CO, USA; Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA; Department of Pediatrics, University of Colorado Denver and Health Sciences Center, Children’s Hospital, Aurora, CO, USA; Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, USA

Funding source(s)

This work is supported by grants from the Cystic Fibrosis Foundation and NIH(U01 HL081335–01, P01DK078669, and the NIH/CU Molecular Biophysics Training Program T32GM065103).

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