Self-transcribing active regulatory region sequencing data analysis software tools
STARR-seq is a massively parallel reporter assay to identify transcriptional enhancers directly based on their activity in entire genomes and to assess their activity quantitatively. Enhancer activity is directly linked to the underlying DNA sequence and measured as presence of the resulting reporter transcripts among cellular RNA by deep sequencing.
Clones downstream candidate sequences of a minimal promoter, such that enhancers will stimulate their own transcription. STARR-seq’s ectopic enhancer–activity assays provide a complementary approach to current chromatin-based methods of identifying genomic enhancers. An analysis has been realized on a fly genomic DNA library in S2 cells, and~5,500 enriched sequences exhibiting a range of enhancer strengths has been identified. Validation of representative subsets with luciferase reporters confirmed the predicted strong and weak enhancer activity of the STARR-seq isolates.
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