Contains a comprehensive characterization of subcellular localization and topology of the complete proteome of Escherichia coli. Two widely used E. coli proteomes (K-12 and BL21) are presented organized into thirteen subcellular classes. STEPdb exploits the wealth of genetic, proteomic, biochemical, and functional information on protein localization, secretion, and targeting in E. coli, one of the best understood model organisms. Subcellular annotations were derived from a combination of bioinformatics prediction, proteomic, biochemical, functional, topological data and extensive literature re-examination that were refined through manual curation.
Laboratory of Molecular Bacteriology; Rega Institute, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium
STEPdb funding source(s)
This work was supported by the European Commission the Operational Programme “Competitiveness and Entrepreneuriship” (OPCE II - EPAN II) - National Strategic Reference Framework (NSRF 2007–2013) [grant codes: 09SYN-13–705 and 09SYN-13–832], an Excellence grant (#1473 to A.E.; General Secretariat of Research), and the Onassis foundation predoctoral program.