Stimfit

[…] and 0%–0.2% biocytin, ph adjusted to 7.3 with koh. 50–100 μm alexa fluor 488 (invitrogen) was added to the internal solution for all somatic recording electrodes., analysis was performed using stimfit 0.9.2–0.13.2 (), clampfit 9 (molecular devices), origin 2015 (microcal), excel (microsoft), and minitab 17 (minitab). ap amplitude was measured from threshold (50 v s−1), and half-duration […]

[…] x-100% and 0.2% streptavidin-cy3 in pbs and were again incubated overnight in 4°c. finally, slices were mounted with dako s3023 medium., custom-made routines written in igor pro 6.3 (wavemetrics), stimfit 0.15 () or prism (graphpad) were used for data analysis and statistical testing. spike threshold value was determined as the time point at which the derivative of voltage exceeded 40 v/s […]

[…] for further optimization by the levenberg-marquardt algorithm., throughout the paper, for clear presentation the stimulus artefact was blanked out. slice electrophysiology data was analysed in stimfit v0.13 (https://github.com/neurodroid/stimfit/wiki/stimfit) using custom python modules (see )., organotypic slices were prepared, transfected, dialysed and pre-treated with biotin-binding […]

[…] normality was tested by the shapiro–wilk test. differences were considered statistically significant at p < 0.05. intrinsic properties as well as psc amplitudes and kinetics were analyzed using stimfit software (). for data acquired with patch-clamp recording (,) or in vivo single-unit recording (see ); n is the number of neurons, with one cell per brain slice (to enable anatomical […]

[…] slices obtained from at least three different animals from three different pups; recordings from virally transduced and non-infected control cells were performed in the same slices., data analysis. stimfit 0.9 software and igor pro (wavemetrics, usa) were used to analyse data from spontaneous and evoked epscs. the rise time 20–80% was determined as the time interval between the points […]