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String Graph Assembler SGA

Assembles large genomes from high coverage short read data. SGA is designed as a modular set of programs, which are used to form an assembly pipeline. SGA implements a set of assembly algorithms based on the FM-index. As the FM-index is a compressed data structure, the algorithms are very memory efficient. The SGA assembly has three distinct phases. The first phase corrects base calling errors in the reads. The second phase assembles contigs from the corrected reads. The third phase uses paired end and/or mate pair data to build scaffolds from the contigs. The output of this software is a PDF report that allows the properties of the genome and data quality to be visually explored. By providing more information to the user at the start of an assembly project, this software will help increase awareness of the factors that make a given assembly easy or difficult, assist in the selection of software and parameters and help to troubleshoot an assembly if it runs into problems.

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SGA classification

SGA specifications

Software type:
Package/Module
Restrictions to use:
None
Computer skills:
Advanced
Maintained:
Yes
Interface:
Command line interface
Operating system:
Unix/Linux
Stability:
Stable

SGA distribution

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SGA support

Documentation

Maintainer

  • Jared Simpson <>

Additional information

https://github.com/jts/sga/wiki/FAQ

Credits

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Publications

Institution(s)

Ontario Institute for Cancer Research, Toronto, Canada

Funding source(s)

This work was supported by the Wellcome Trust Sanger Institute (Wellcome Trust grant 098051) and by the Ontario Institute for Cancer Research through funding provided by the Government of Ontario.

Link to literature

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