An ImageJ plug-in based on the concept of nematic tensor, which can provide a quantitative description of the anisotropy of fiber arrays and their average orientation in cells, directly from raw images obtained by any form of microscopy. FibrilTool has been validated on microtubules, actin and cellulose microfibrils, but it may also help analyze other fibrillar structures, such as collagen, or the texture of various materials. The tool is ImageJ-based, and it is therefore freely accessible to the scientific community and does not require specific computational setup. The tool provides the average orientation and anisotropy of fiber arrays in a given region of interest (ROI) in a few seconds.
Infers the preferred orientation of structures present in an input image. Directionality is a plugin for the image analysis software ImageJ. It computes a histogram indicating the amount of structures in a given direction. The interface allows to specify: (i) the number of bins, (ii) the starting angle for analysis, (iii) the debug flag, (iv) the result table flag and (v) the orientation map flag.