Provides an integrated computational solution designed specifically for large-scale RNA structure mapping and reconstruction across any transcriptome. StructureFold automates the processing and analysis of raw high-throughput RNA structure profiling data, allowing the seamless incorporation of wet-bench structural information from chemical probes and/or ribonucleases to restrain RNA secondary structure prediction via the RNAstructure and ViennaRNA package algorithms. StructureFold performs reads mapping and alignment, normalization and reactivity derivation, and RNA structure prediction in a single user-friendly web interface or via local installation. StructureFold is freely available as a component of Galaxy.
Department of Biology, Center for RNA Molecular Biology, Bioinformatics and Genomics Graduate Program,Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA, USA; Galaxyprojectorg, University Park, PA, USA and Baltimore, MD, USA