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cmeAnalysis / clathrin-mediated endocytosis analysis

Allows quantification of clathrin-coated pit dynamics from fluorescence time-lapse data. cmeAnalysis provides functionalities including: (1) sensitive detection, (2) tracking (based on u-track), (3) master/slave detection for multi-channel data, (4) intensity-based classification of coated structures, and (5) lifetime analysis. It also contains a graphical user interface (GUI) for inspection of analysis results from individual movies.

Automated Image Analysis of Protein Localization in Budding Yeast

Computational methods were developed to automatically analyze the images created by the University of California, San Francisco (UCSF) yeast GFP fusion localization project. The automated method provides an objective, quantitative and repeatable assignment of protein locations that can be applied to new collections of yeast images (e.g. for different strains or the same strain under different conditions). It is also important to note that this performance could be achieved without requiring colocalization with any marker proteins.