Suite2p

[…] running Ubuntu 17.04 with a six core Intel Core i7-6800 K [email protected] GHz). FISSA is thus well suited for processing large datasets with long imaging periods. However, as opposed to other methods (cNMF, suite2P), FISSA does not automatically segment the images into defined ROIs; therefore, the total time for data processing also depends on the method used for defining ROIs as well as on the data load […]

[…] the power at sample was controlled in the range 30-40 mW. Data analysis was performed in ImageJ and MATLAB and was restricted to cell bodies. Detection of region of interest (ROI) was performed with Suite2p (). The relative changes in fluorescence were calculated as dF/F0 = (F(t)-F0)/(F0). Orientation tuning curves were generated by taking the mean response for each orientation during the entire […]

[…] urface. This might explain why the number of detected circles increased as the imaging depth increased to 1000 µm ().Motion correction for calcium imaging was performed by phase-correlation using the Suite2P package (, http://github.com/cortex-lab/Suite2P). After the motion correction, images were three frame-averaged before being analyzed. A constrained non-negative matrix factorization (cNMF) al […]