Super resolution imaging analysis software tools | Bioimaging

In recent years, super-resolution optical imaging techniques have been developed to overcome the diffraction limit in fluorescence microscopy (Hell, 2007; Huang et al. 2010). Among these techniques, methods based on stochastic switching and localization of individual molecules, such as stochastic optical reconstruction microscopy (STORM) (Rust et al. 2006) and (fluorescent) photoactivated localization microscopy ((F)PALM) (Betzig et al. 2006; Hess et al. 2006), achieve sub-diffraction-limit resolutions by sequentially imaging and localizing fluorescent emitters in the sample.

Source text:
(Babcock et al., 2012) A high-density 3D localization algorithm for stochastic optical reconstruction microscopy. Opt Nanoscopy.

Image preprocessing

Image processing

Structural analysis

Dynamic analysis

Advanced fluorescence microscopy

Image data management and annotation