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Super-resolution optical imaging | Bioimaging software tools

In recent years, super-resolution optical imaging techniques have been developed to overcome the diffraction limit in fluorescence microscopy (Hell, 2007; Huang et al. 2010). Among these techniques, methods based on stochastic switching and localization of individual molecules,…

Super-resolution imaging steps

Image preprocessing

Image processing

Structural analysis

Dynamic analysis

BioImageXD
Desktop

BioImageXD

Analyzes, processes and visualizes multi-dimensional microscopy images.…

Analyzes, processes and visualizes multi-dimensional microscopy images. BioImageXD puts open-source computer science tools for three-dimensional visualization and analysis into the hands of all…

DAOSTORM
Desktop

DAOSTORM

Provides a more quantitative report of spatial distribution of fluorescent…

Provides a more quantitative report of spatial distribution of fluorescent molecules, to increase quality of super-resolved images of biological samples and to maintain performance at high imaging…

SIMToolbox
Desktop

SIMToolbox

An open-source, modular set of functions for MATLAB equipped with a user…

An open-source, modular set of functions for MATLAB equipped with a user friendly graphical interface and designed for processing 2D and 3D data acquired by structured illumination microscopy. Both…

iNMF
Desktop

iNMF Non-negative matrix factorisation with iterative restarts

Models a noisy dataset of highly overlapping fluorophores with intermittent…

Models a noisy dataset of highly overlapping fluorophores with intermittent intensities. iNMF enlarges the family of localisation microscopy techniques, and enables using quantum dots as fluorescent…

MIB
Desktop

MIB Microscopy Image Browser

Serves for effective segmentation of multidimensional datasets. MIB can…

Serves for effective segmentation of multidimensional datasets. MIB can recognize several number of imaging formats and offers a variety of image processing tools. It also simplifies utilization and…

3D-DAOSTORM
Desktop

3D-DAOSTORM

An extension of the DAOPHOT analysis method to the analysis of…

An extension of the DAOPHOT analysis method to the analysis of astigmatism-based 3D super-resolution data where the z position of the emitter is encoded in the x and y width of its point spread…

MetaMorph
Desktop

MetaMorph

It is the ideal "glue" for easily integrating dissimilar fluorescent…

It is the ideal "glue" for easily integrating dissimilar fluorescent microscope hardware and peripherals into a single custom workstation, while providing all the tools needed to perform…

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