TALOS+ statistics

Tool stats & trends

Looking to identify usage trends or leading experts?


TALOS+ specifications


Unique identifier OMICS_03404
Alternative name Torsion Angle Likeliness Obtained from Shift and Sequence Similarity
Interface Web user interface
Restrictions to use None
Output data The predictions for individual residues with missing chemical shifts, and the secondary structure.
Computer skills Basic
Stability Stable
Registration required Yes
Maintained Yes


  • person_outline Yang Shen
  • person_outline Ad Bax

Additional information

https://spin.niddk.nih.gov/NMRPipe/talos/ TALOS+ is now also installed as part of the NMRPipe System.

Publications for Torsion Angle Likeliness Obtained from Shift and Sequence Similarity

TALOS+ citations


In silico optimization of a guava antimicrobial peptide enables combinatorial exploration for peptide design

Nat Commun
PMCID: 5902452
PMID: 29662055
DOI: 10.1038/s41467-018-03746-3

[…] using the calibration by Hyberts et al.. The angle restraints of phi and psi of the protein backbone dihedral angles were predicted based on analysis of 1Hα and 13Cα chemical shifts using the program TALOS+. Several cycles of XPLOR were performed using standard protocols. After each cycle rejected restraints, side-chain assignments, NOEs, and dihedral violations were analyzed. Two hundred structur […]


LyeTxI b, a Synthetic Peptide Derived From Lycosa erythrognatha Spider Venom, Shows Potent Antibiotic Activity in Vitro and in Vivo

Front Microbiol
PMCID: 5897548
PMID: 29681894
DOI: 10.3389/fmicb.2018.00667
call_split See protocol

[…] tained were 2.8, 3.4, and 5.0 Å (strong, medium, and weak NOE, respectively). Forty-four dihedral angle restrains were obtained from analysis of Cα, Hα, Cβ, N, and HN chemical shifts with the program TALOS+ (). Structure calculations were performed using the Xplor-NIH software, version 2.27 (). A total of 100 structures, starting with an extended conformation, were generated using a simulated anne […]


NMR Structure, Dynamics and Interactions of the Integrin β2 Cytoplasmic Tail with Filamin Domain IgFLNa21

Sci Rep
PMCID: 5882645
PMID: 29615775
DOI: 10.1038/s41598-018-23866-6
call_split See protocol

[…] Hybrid IgFLNa21 protein backbone φ and ψ torsion angles were predicted using TALOS+ server. Crosspeaks from 13C-HSQC-NOESY and 15N -HSQC-NOESY, were selected and integrated using SPARKY. Distance constraint and structure calculation were calculated using CYANA 2.1 automated NO […]


Structural and Functional Insights into Bacillus subtilis Sigma Factor Inhibitor, CsfB

PMCID: 5890618
PMID: 29526435
DOI: 10.1016/j.str.2018.02.007

[…] ESY spectra (NOEs from the filtered NOESY experiment were defined as intermolecular while the NOEs in the other NOESY experiments were treated as ambiguous) and dihedral angle restraints estimated by TALOS+ (). Typical annealing parameters were used for distance (10, 15, 50 and 100 Kcal/mol for high temperature, initial cool1, final cool1 and cool2 force constants) and dihedral restraints (50, 150 […]


Solution structure and dynamics of anti CRISPR AcrIIA4, the Cas9 inhibitor

Sci Rep
PMCID: 5832863
PMID: 29497118
DOI: 10.1038/s41598-018-22177-0

[…] raints were derived from the NOE spectra and classified into distance ranges according to the peak intensity. ϕ/ψ torsion angle restraints were derived from backbone chemical shifts using the program TALOS+. The solution structure of AcrIIA4 was calculated by simulated annealing in torsion angle space using the Xplor-NIH program. The target function of simulated annealing included a covalent geome […]


Physical basis of amyloid fibril polymorphism

Nat Commun
PMCID: 5816019
PMID: 29453354
DOI: 10.1038/s41467-018-03164-5

[…] to 0 °C. All experiments used 100 kHz of 1H decoupling using SPINAL-64 and 10 kHz of MAS spinning. Spectra were analyzed with CcpNmr Analysis 2.4.0. Secondary structure analysis was accomplished with TALOS+ based on the 13Cα, 13Cβ, 13C backbone carbonyl, and 15N backbone amide chemical shifts (Supplementary Table ). The analyzed fibrils were formed under conditions identical to our cryo-EM samples […]


Looking to check out a full list of citations?

TALOS+ institution(s)
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
TALOS+ funding source(s)
Supported by the Intramural Research Program of the NIDDK, NIH.

TALOS+ reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review TALOS+