Yields a set of coding sequences (CDSs) whose nucleotide sequences are as different as possible and whose codon usage frequencies are as highly adapted as possible to the host organism. Tandem Designer is a method for designing a set of CDSs that are not only unlikely to induce homologous recombination when integrated into a genome, but also have highly adapted codon usage frequencies. This platform optimizes sequences to increase nucleotide differences among CDSs that encode the same target protein.
Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan; INTEC Inc, Yokohama, Japan; Graduate School of Science and Technology, Hirosaki University, Hirosaki, Japan; Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan
Tandem Designer funding source(s)
Partially supported by the commission for the project focused on developing key technology of discovering and manufacturing drugs for next-generation treatment and diagnosis from the Japan Agency for Medical Research and Development (AMED).