TANGO protocols

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TANGO specifications


Unique identifier OMICS_06601
Alternative name Tools for Analysis of Nuclear Genome Organization
Software type Framework/Library
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java, R
License GNU General Public License version 2.0
Computer skills Medium
Stability Stable
Maintained Yes


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  • person_outline Thomas Boudier <>

Publication for Tools for Analysis of Nuclear Genome Organization

TANGO in pipeline

PMCID: 4565980
PMID: 26312502
DOI: 10.7554/eLife.07759.019

[…] and wavelength alignment was completed using the softworx (ge healthcare) program. 3d image rendering and analysis was performed using imaris version 7.6 (bitplane an oxford instruments company), tango () and 2d image analysis using fiji (), and cellprofiler ()., data and statistical analyses were performed using excel and graphpad prism software. results are shown as mean ± s.e.m/sd and box […]

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TANGO in publications

PMCID: 5913236
PMID: 29686427
DOI: 10.1038/s41467-018-04088-w

[…] freely available from the author’s github repo at https://github.com/prnicovich/pavest.git., nuclear translocation of transcription factors nfat and tsad was determined by using imagej/fiji plugin tango and its included nuclear edge detector for nuclear segmentation and determining integrated intensity density., vesicle tracking: we used diatrack 3.04 pro[vallotton et al tritrack]. at first, […]

PMCID: 5547466
PMID: 28784182
DOI: 10.1186/s13072-017-0146-0

[…] plotting. for a detailed description, see []., nearest-neighbor/minimal distance measurements between centroid xyz coordinates of differently labeled segmented fish ‘objects’ were taken using the tango plugin for imagej/fiji [, , ]. mode-subtracted, 16-bit transformed 3d-sim image stacks were imported into tango. xyz centroid coordinates from segmented objects were extracted based […]

PMCID: 4914320
PMID: 26799419
DOI: 10.18632/oncotarget.6938

[…] fluorescence images (45 per nucleus) at 0.2 μm intervals. following deconvolution, a minimum of 50 nuclei from each treatment group were processed for colocalization using nih image j software and tools for analysis of nuclear genome organization (tango) software []. all structures were segmented using the stock segmentation; background was removed for fitc and cy3 signals with a tophat […]

PMCID: 4659165
PMID: 26607933
DOI: 10.1186/s12859-015-0814-7

[…] solutions on the same hand-segmented stacks we used to assay our pipeline. we initially tested a large number of possibilities (including icy [], farsight [], ilastik [], fiji and trainable weka [], tango [], bioimagexd [], and v3d [, ]) and focused on four that appeared particularly promising for our image data: imaris (bitplane), vaa3d gradient vector field (gvf) plugin [], ilastik [], […]

PMCID: 4571307
PMID: 25947134
DOI: 10.1091/mbc.E14-05-1002

[…] (invitrogen). these beads also allowed us to measure the maximum resolution to be 0.2 μm in x/y and 0.5 μm in z. each field usually contained 10–20 nuclei., images were automatically processed using tango, software we previously developed (). nuclei were automatically segmented using a home-made 3d watershed-derived algorithm included in tango (called a nucleus edge detector), which detects […]

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TANGO institution(s)
CNRS UMR7196, INSERM U565, MNHN, Paris, France

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