TargetScan

[…] The target genes of the five-miRNA (miR-203, miR-424, miR-1266, miR-1293, and miR-4772) were predicted using TargetScan, miRDB, miRTarBase, and miRanda online analysis tools. To further enhance the reliability of the bioinformatic analysis, the overlapping target genes were identified using a Venn diagram. U […]

[…] d two relatively closely related species: D. simulans and D. yakuba. miR310s CDS targets are conserved between D.melanogaster and D.simulans. Transcripts targeted by miR310s were identified using the TargetScan algorithm [, , , ]. 3’UTR annotation was extracted from BDGP6.83. The whole genome alignments were dm6, droSim1, and droYak3 available at the UCSC Genome Browser. miRNA expression during th […]

[…] e list of predicted genes cancer datasets from TCGA database was analyzed and downloaded using cBioportal and ChIPBase v2. online tools. Predictions of miRNA targets were performed and retrieved from TargetScan, miRanda, miRcode, Starbase and DIANA online prediction tools. For prediction of RNA-RNA interactions and RNA-RNA binding protein (RBPs) interaction, IntaRNA, and CLIPdb online tools were e […]

[…] ersely expressed to the altered miRNAs. There were no correlations between the number of binding sites in the 3’-UTR region and the mRNA fold changes. It has to be noticed that our analyses, based on TARGETSCAN predictions, did not take into account miRNA binding sites in the coding region of mRNAs. Highly targeted genes involved in TGF-beta and IGF-I pathways and miRNA functioning were predicted […]

[…] P-wild not in MT1JP-mut in both SGC-7901 and BGC-823 cell lines, which indicated that miR-92a-3p is a target of lncRNA MT1JP (Fig. ). Further prediction of target genes of miR-92a-3p was performed by TargetScan (http://www.targetscan.org/vert_71/) and microRNA.org (http://www.microrna.org/microrna/home.do), and FBXW7 was selected as the target gene of miR-92a-3p for it acquired consistently highes […]

[…] ious study, HSP27 was reported to be an miR-577 target. Thus, we investigated whether a similar ceRNA mechanism exists among DANCR, HSP27 and miR-577. Using the online theoretical prediction programs targetscan and lncbase, we found that DANCR and HSP27 shared the same miR-577 binding sites (at the transcription positions 424-430 in DANCR and 77-83 in HSP27) (Fig. ). Next, we confirmed that miR-57 […]