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Homology-based taxonomic classification software tools | 16S ribosomal RNA sequencing data analysis

The taxonomic classification of environmental 16S rRNA gene sequences is carried out by using either a homology-based or prediction-based approach. The former approach requires the alignment of a query 16S rRNA sequence with all the 16S rRNA sequences present in the reference database (Jonasson et al., 2002), such as Ribosomal Database Project (Cole et al., 2009), Greengenes (DeSantis et al., 2006) and SILVA (Pruesse et al., 2007). Source text: Chaudhary et al., 2015.

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