The first human inosinome atlas comprising 3,041,422 Adenine to Inosine events identified in six tissues from three healthy individuals. Matched directional total-RNA-Seq and whole genome sequence datasets were generated and analysed within a dedicated computational framework, also capable of detecting hyper-edited reads. Inosinome profiles are tissue specific and edited gene sets consistently show enrichment of genes involved in neurological disorders and cancer. Overall frequency of editing also varies, but is strongly correlated with ADAR expression levels.
Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Bari, Italy; Institute of Biomembranes and Bioenergetics, National Research Council, Bari, Italy; National Institute of Biostructures and Biosystems, Rome, Italy; Center of Excellence in Comparative Genomics, University of Bari, Bari, Italy
The RNA Editing ATLAS funding source(s)
This work was supported by the Italian Ministero dell’Istruzione, Università e Ricerca: PRIN 2009, 2010 and 2012; Consiglio Nazionale delle Ricerche: Flagship Project Epigen, Medicina Personalizzata and Aging Program 2012-2014; Italian Ministry for Foreign Affairs: Italy-Israel actions.