ThunderSTORM specifications


Unique identifier OMICS_06526
Name ThunderSTORM
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java
License GNU General Public License version 2.0
Computer skills Medium
Stability Beta
Maintained Yes


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  • person_outline Tomas Lukes <>
  • person_outline Guy Hagen <>

Additional information

Publications for ThunderSTORM

ThunderSTORM in publications

PMCID: 5821895
PMID: 29467364
DOI: 10.1038/s41467-018-03127-w

[…] or bivariate-) pcf values at each scale., data acquired by single-molecule localization microscopy was analysed by the n-storm module in nis-elements (nikon) or a previously described algorithm (thunderstorm) for the identification of individual peaks and group them into functions that reflect the positions of single molecules. next, peaks were grouped and assigned to individual molecules […]

PMCID: 5841371
PMID: 29361123
DOI: 10.1093/gigascience/giy002

[…] for imaging alexa 532, we used a 1-w, 532-nm laser (dragon laser) and an appropriate fluorescence emission filter (569–610 nm, chroma), as previously described []., we analyzed the data using thunderstorm [, ] with the default settings. the default settings involve use of a wavelet-based filter for feature enhancement [], followed by local maximum detection of single molecules […]

PMCID: 5821461
PMID: 29465397
DOI: 10.7554/eLife.32057.041

[…] plane of focus were obtained for ezra-eyfp (), ftsz-eyfp () and ezra-meyfp (). the mean localisation precision of eyfp was calculated using two different formulae: the ‘thompson equation’ () by the thunderstorm imagej plugin yielded 24 (s.d. 8.5) nm while a using a modified version of this equation () yielded 27 (s.d. 8.7) nm. we also measured it experimentally using nearest neighbour […]

PMCID: 5711914
PMID: 29196704
DOI: 10.1038/s41598-017-16834-z

[…] aging period. monitoring of lateral drift was performed with either 0.1 µm 540/560 fluospheres (thermofisher) as fiducial markers or through cross-correlation. reconstructions were performed with the thunderstorm software package using the wavelet filter and maximum likelihood estimator fitting. cpca-pcb nanopatterns were imaged with a 514 nm coherent sapphire laser at a power density of 1.6 kw/cm […]

PMCID: 5705721
PMID: 29184084
DOI: 10.1038/s41598-017-16570-4

[…] at 100 fps on a ~50 × 50 μm wide field of view, which ensured adequate reconstruction of super-resolved images of single whole cells. the diffraction limited data were subsequently analysed using thunderstorm, an imagej plugin developed for storm microscopy. lateral thermal drift was corrected using thunderstorm. poor localisations, based on their positional uncertainty and fitted sigma […]

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ThunderSTORM institution(s)
Laboratoire d’Optique Biomédicale, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland; Department of Radioelectronics, Faculty of Electrical Engineering, Czech Technical University in Prague, Prague, Czech Republic; UCCS center for the Biofrontiers Institute, University of Colorado at Colorado Springs, Colorado Springs, CO, USA
ThunderSTORM funding source(s)
Supported by the UCCS center for the University of Colorado BioFrontiers Institute, by the Czech Science Foundation, by Czech Technical University in Prague (grant number SGS16/167/OHK3/2T/13) and by a SCIEX scholarship (project code 13.183).

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