It is based on the recently developed Global Translation Initiation sequencing (GTI-seq) technology, which provides global mapping of TIS codons at nearly single-nucleotide resolution. GTI-seq has the potential to reveal a comprehensive and unambiguous set of TIS codons across the entire transcriptome. TISdb provides tools to search for TIS positions and the associated open reading frames (ORFs) based on multiple GTI-seq datasets. Some filters are available to gain different reliability of the results. Flexible search engine offers different input format. Result output includes table coordinates and images for easy visualization.