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We use the term Tn-seq to refer to a range of transposon insertion sequencing techniques that use a random transposon mutant library and high-throughput sequencing to study fitness of mutant strains and/or to identify genes that are essential or advantageous for growth under a specific set of conditions. Thanks to the novel advances in deep sequencing technologies, this technique has become useful for understanding gene function and the genetics behind microbial physiology. (Gawronski et al., 2009; Sarmiento et al., 2013; van Opijnen et al., 2009; van Opijnen and Camilli, 2013; Gallagher et al., 2011; Fu et al., 2013). Source text: Solaimanpour et al., 2015.