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Tomogram/Subtomogram averaging software tools | Cryo-electron tomography image analysis

Analogous to single-particle reconstruction in cryo-electron microscopy, structures present in multiple copies within tomograms can be extracted, aligned, and averaged, thus increasing the signal-to-noise ratio and resolution. This reconstruction approach, termed subtomogram averaging, can be used to determine protein structures in situ. It can also be applied to facilitate more conventional 2D image analysis approaches.

Source text:
(Wan et al., 2016) Cryo-Electron Tomography and Subtomogram Averaging. Methods Enzymol.

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