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Topology-based pathway analysis software tools | High-throughput sequencing

The goal of pathway analysis is to identify the pathways significantly impacted in a given phenotype. Many current methods are based on algorithms that consider pathways as simple gene lists, dramatically under-utilizing the knowledge that such pathways are meant to capture. During the past few years, a plethora of methods claiming to incorporate various aspects of the pathway topology have been proposed. These topology-based methods, sometimes referred to as "third generation," have the potential to better model the phenomena described by pathways.

Source text:
(Mitrea et al., 2013) Methods and approaches in the topology-based analysis of biological pathways. Front Physiol.

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